KARPAS 422

06101702, human lymph (lymphoma). Round polygonal singlets or small clusters in suspension

• 別名: KARPAS, 422
• UNSPSCコード: 41106514

特性

• product name: KARPAS 422, from human lymph(lymphoma), 06101702
• 由来生物: human lymph (lymphoma)
• 成長モード: Suspension
• 核型: Hyperdiploid with 10% polyploidy
• 形態: Round polygonal cells growing singly or in small clusters in suspension
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice

詳細

細胞株の由来

Human B cell non-Hodgkin lymphoma

細胞株の説明

Established from a pleural effusion of 73 year-old woman diagnosed with B-cell non-Hodgkin lymphoma (intra-abdominal, diffuse large cell lymphoma, refractory, terminal). Carries t(14;18) IGH-BCL2 fusion gene (break point in major breakpoint region, MBR).

DNAプロファイル

特記事項なし

培地

RPMI 1640 + 2 mM Glutamine + 20% Fetal Bovine Serum (FBS).

継代と培養方法

Maintain cultures between 0.5 - 2 x 1000,000 cells/ml; 5% CO₂; 37 °C. Doubling time approximately 60-90 hours. Split saturated culture 1:2 every 2-4 days. Round polygonal cells growing singly or in small clusters in suspension.

その他情報

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免責事項

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.