COS-7-InVitrus

5063001, African green monkey kidney, Fibroblast

• UNSPSCコード: 41106514

特性

• product name: COS-7-InVitrus, 05063001, from African green monkey kidney, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• 由来生物: African green monkey kidney
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 受容体: Not specified

詳細

細胞株の由来

Monkey African green kidney, SV40 transformed, serum-free

細胞株の説明

COS-7 cell line (Sigma catalog no. 87021302) adapted to grow in the chemically defined protein- and peptide-free InVitrus medium (Cell Culture Technologies). This cell line is substrate dependent, i.e., adherent. COS-7 was derived from CV-1 simian cells transformed by an origin-defective mutant of SV40.

培地

InVitrus medium (Cell Culture Technologies http://www.cellculture.com/) + 4 mM L-Glutamine (G7513) + 5 ug/ml Insulin. When freezing cells down use culture medium (50:50 conditioned medium:fresh medium) + 10% DMSO (D2650).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:2, i.e., seeding at 5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Please note: As the cell culture medium does not contain protein it will not inactivate trypsin. Once cells have detached from the flask it is necessary to dilute the trypsin with the cell culture medium, centrifuge the cells and resuspend in fresh medium. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.

その他情報

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