RCC4 plus vector alone

3112702, human kidney, Epithelial

• 別名: Human RCC4, RCC4 Cell Line
• UNSPSCコード: 41106514

特性

• product name: RCC4 plus vector alone, 03112702
• 由来生物: human kidney
• 成長モード: Adherent
• 核型: Not specified
• 形態: Epithelial
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Renal cell carcinoma cell line RCC4 stably transfected with an empty expression vector, pcDNA3, conferring neomycin resistance.

細胞株の説明

Renal cell carcinoma cell line RCC4 stably transfected with an empty expression vector, pcDNA3, conferring neomycin resistance. This cell line is VHL-deficient i.e. the von Hippel-Lindau tumour suppressor gene product is not expressed. These cells are tumourigenic.

アプリケーション

RCC4 plus vector alone is mainly used to study the tumour suppressor gene product function - von Hippel-Lindau tumour suppressor gene. It is also used in cell responses to hypoxia.

• RCC4 plus vector alone has been used in study involving the effect of in vitro ischemia on canonical hypoxic HIF-1α (Hypoxia Inducible Factor α) stabilization.
• It has been used in the study involving CMA (chaperone-mediated autophagy)-mediated degradation of HIF1α in the lysosome.
• It has also been used to study lipodepsipeptide MDN-0066-mediated inhibition of VHL/HIF (Von Hippel-Lindau/hypoxia inducible factor) pathway.

DNAプロファイル

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 12
D7S820: 8.2
THO1: 9.3
TPOX: 11
vWA: 16

培地

DMEM + 10% FBS + 2 mM Glutamine + G418 0.5mg/ml

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% Trypsin/EDTA; 5% CO₂; 37°C.

その他情報

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