P3X63Ag8.653-TurboDoma

3092502, mouse blood, Lymphoblast

• UNSPSCコード: 41106514

特性

• 製品名: P3X63Ag8.653-TurboDoma, 03092502
• 由来生物: mouse blood
• 成長モード: Suspension
• 核型: Not specified
• 形態: Lymphoblast
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 関連疾患: cancer
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Mouse BALB/c myeloma, serum-free

細胞株の説明

P3X63Ag8.653 cell line (Sigma Catalogue number. 85011420) adapted to grow in the chemically defined protein- and peptide-free medium TurboDoma^™. Suitable for use as a fusion parent for serum-free generation of hybridomas.

アプリケーション

Fusion partner for generation of serum-free hybridomas.

培地

TurboDoma^™ medium + 4mM Glutamine. When freezing cells down use TurboDoma medium (50:50 conditioned medium:fresh medium) + 4mM Glutamine + 10% DMSO.

継代と培養方法

Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 10⁵ cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO₂; 37°C.

その他情報

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法的情報

TurboDoma is a trademark of Cell Culture Technologies