IIC9 (CH0)

00030814

• UNSPSCコード: 41106514

特性

• 由来生物: hamster embryo
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast
• 製品: Not specified
• 受容体: PAR-1 (protease-activated receptor 1, thrombin receptor), EGF, PDGF
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Chinese hamster embryo fibroblast

細胞株の説明

The Chinese hamster embryo fibroblast (CHEF) line is a nontumorigenic and stable diploid cell line, with a high serum requirement for growth. It is isolated from embryo preparation and is a spontaneous tumorigenic anchorage-independent line.

アプリケーション

IIC9 (CH0) is suitable for selective growth of some rodent epithelial cells in a medium containing citrulline.

培地

DMEM:F12 (1:1) (D8062) + 5% FBS / FCS (F2442) + 2mM L-Glutamine (G7513).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:10 ie. seeding at 2-4 x10,000 cells/cm² using 0.25% trypsin /EDTA; 37°C; 5% CO₂.

その他情報

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