CHSE-214

21714, fish embryo, Fibroblast-like

• 別名: CHSE/F
• UNSPSCコード: 41106514

特性

• product name: CHSE-214, 00021714
• 由来生物: fish embryo
• 成長モード: Adherent
• 核型: Not specified
• 形態: Fibroblast-like
• 製品: Not specified
• 受容体: Not specified
• テクニック: cell culture | mammalian: suitable
• 輸送温度: dry ice
• 保管温度: −196°C

詳細

細胞株の由来

Common bluegill (Lepomis macrochirus), normal

細胞株の説明

The fish line CHSE/F was derived from Common bluegill (Lepomis macrochirus) embryo. It is susceptible to various fish viruses including infectious hematopoietic necrosis virus (IHNV); viral haemorrhagic septicaemia virus (VHSV); spring viraemia of carp virus (SVCV); and infectious pancreatic necrosis virus (IPNV).
ECACC has been advised by the CCLV that this cell line is derived from Lepomis macrochirus (Common bluegill) not Oncorhynchus tshawytscha (Chinook salmon) as originally reported. This has been confirmed by DNA sequencing at ECACC. The CCLV have changed the cell line name to CHSE/F to indicate this. The line is used in viral diagnostics and gives better results than BF-2 (Sigma Catalogue number 00021712), a commonly used cell line of Lepomis macrochirus. The sequence of both lines (BF-2 and CHSE/F possess the same nucleotide exchange but are different in culture behaviour and in virus propagation.

アプリケーション

Fish virus studies

培地

EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 1% Non Essential Amino Acids(NEAA) (M7145) + 120mg/l Sodium Pyruvate(NaP) (S8636) + 10% FBS / FCS (F2442).

継代と培養方法

Split sub-confluent cultures (70-80%) 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA; grow cells in dark (e.g. wrapped in foil) at 20-26ºC with 26ºC optimum temperature; monolayer is stable for at least 10 days with maintenance medium containing 1-2% FBS.

その他情報

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