由来生物
mouse
品質水準
結合体
unconjugated
抗体製品の状態
ascites fluid
抗体製品タイプ
primary antibodies
クローン
2H2A7C2, monoclonal
分子量
antigen 80 kDa
交差性
pig, rat, bovine, human
テクニック
indirect immunofluorescence: 1:25
western blot: 1:1,000
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
遺伝子情報
human ... CAPN1(823)
rat ... Capn1(29153)
詳細
Calpain-1 catalytic subunit is a protein encoded by the CAPN1 gene in humans. Calpains are calcium dependent proteases constituting a family of proteins. They share a homologous cysteine-protease domain and an E-F hand Ca2+-binding domain. The calpain system consists of two ubiquitous forms of calpain (m-calpain and μ-calpain), a tissue specific calpain (n-calpain) and a calpain inhibitory protein (calpastatin).
特異性
エピト-プマッピングの研究から、エピト-プはヒトμ-カルパインのアミノ酸245-265(ドメインII)の間にあることが示されています。μ-カルパインと特異的に反応する抗体です。m-カルパイン, n-カルパインやカルモジュリン、カルパスタチンとは反応しません。免疫沈降には適していません。イムノブロッティングにおいて、ヒト血小板, 赤血球、ウシ血小板, 心臓 ,骨格筋や、ラット筋芽細胞 ,腎臓 ,肝臓 ,脾臓サンプルとの反応性が観察されています。ブタLLC-PK1細胞の免疫蛍光染色では、細胞質が広く染色された様子が観察されています。
免疫原
ウシ骨格筋由来μ-カルパイン。
アプリケーション
Monoclonal Anti-μ-Calpain (Domain II) antibody produced in mouse is suitable for indirect immunofluorescence at a dilution of 1:25 and western blotting at a dilution of 1:1000.
生物化学的／生理学的作用
Calpain is involved in calmodulin-independent pathway for the activation of calcineurin. Endogenous calpain I forms active calcineurin in the human heart by proteolysis of calcineurin A, which may lead to pathogenesis of myocardial disease. μ-calpain, on overexpression, may have relationship with intractable epilepsy as well as the clinicopathological characteristics in such patients. Its activity may increase in skeletal muscle of gastric cancer patients.
物理的形状
PBS溶液 (0.05%アジ化ナトリウム含有)。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
C267-100UL-PW:
C267-100UL:
C267-BULK:
C267-VAR:
Four genes for the calpain family locate on four distinct human chromosomes.
Ohno S, Minoshima S, Kudoh J, Fukuyama R, Shimizu Y, Ohmi-Imajoh S, Shimizu N, Suzuki K.
Cytogenetics and Cell Genetics (1990)
T Glaser et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(17), 7879-7883 (1994-08-16)
Limited proteolysis by calpain (Ca(2+)-activated protease; EC 3.4.22.17) is believed to regulate the function of membrane enzymes and modify the behavior of membrane structural proteins. Calpain is activated by autolysis. The degradation of band 3 protein by mu-calpain is known
A Ravid et al.
Endocrinology, 135(6), 2822-2825 (1994-12-01)
mu-Calpain is a calcium-dependent neutral thiol protease activated by micromolar concentrations of calcium. mu-Calpain is implicated in various cellular functions regulated by calcium including exocytosis, cell fusion, apoptosis and control of cell proliferation. We studied the effect of 1,25-(OH)2D3 on
R W Neumar et al.
Journal of neurochemistry, 66(1), 421-424 (1996-01-01)
Proteolytic degradation of numerous calpain substrates, including cytoskeletal and regulatory proteins, has been observed during brain ischemia and reperfusion. In addition, calpain inhibitors have been shown to decrease degradation of these proteins and decrease postischemic neuronal death. Although these observations
Takayuki Wakasugi et al.
PloS one, 14(2), e0212889-e0212889 (2019-02-27)
Pulmonary arterial hypertension (PAH) is characterized by remodeling and narrowing of the pulmonary arteries, which lead to elevation of right ventricular pressure, heart failure, and death. Proliferation of pulmonary artery smooth muscle cells (PASMCs) is thought to be central to
資料
Quantitative and qualitative western blotting to validate knockdown by esiRNA. Sigma-Aldrich.com
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