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由来生物
mouse
結合体
biotin conjugate
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
secondary antibodies
クローン
RG-16, monoclonal
形状
buffered aqueous solution
化学種の反応性
rabbit
以下との反応禁止
human
pig, chicken, bovine, horse, human, guinea pig, turkey, rat, canine, goat, feline, sheep
テクニック
direct ELISA: 1:60,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,500
western blot: 1:200,000-1:400,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)
western blot: 1:200,000
アイソタイプ
IgG1
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
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関連するカテゴリー
詳細
Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.
特異性
ウサギIgとのみ結合します。還元されたウサギIgとは反応しません。
アプリケーション
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.
生物化学的／生理学的作用
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. IgM antibodies are present as pentamers in the serum and are produced in response to antigens.
物理的形状
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
B3275-.5ML:
B3275-.2ML:
B3275-BULK:
B3275-VAR:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Takeshi Hayashi et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 23(10), 1117-1128 (2003-10-04)
The endoplasmic reticulum (ER), which plays important roles in apoptosis, is susceptible to oxidative stress. Because reactive oxygen species (ROS) are robustly produced in the ischemic brain, ER damage by ROS may be implicated in ischemic neuronal cell death. We
Milena Penkowa et al.
Journal of neuroscience research, 73(4), 481-496 (2003-08-05)
6-aminonicotinamide (6-AN) is a niacin antagonist, which leads to degeneration of gray matter astrocytes mainly in the brainstem. We have examined the role of interleukin-6 (IL-6) in this degenerative process by using transgenic mice with astrocyte-targeted IL-6 expression (GFAP-IL6 mice).
Takeshi Hayashi et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 23(8), 949-961 (2003-08-07)
Although the endoplasmic reticulum (ER) is implicated in neuronal degeneration in some situations, its role in delayed neuronal cell death (DND) after ischemia remains uncertain. The authors speculated that ER stress is involved in DND, that it is reduced by
M Penkowa et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 20(8), 1174-1189 (2000-08-19)
To study the importance of metallothionein-I and -II (MT-I+II) for brain inflammation and regeneration, the authors examined normal and MT-I+II knock-out (MT-KO) mice subjected to a cortical freeze injury. Normal mice showed profound neurodegeneration, inflammation, and gliosis around the injury
Albert Quintana et al.
Journal of neuroscience research, 82(5), 701-716 (2005-11-04)
Tumor necrosis factor-alpha (TNF-alpha) is one of the mediators dramatically increased after traumatic brain injury that leads to the activation, proliferation, and hypertrophy of mononuclear, phagocytic cells and gliosis. Eventually, TNF-alpha can induce both apoptosis and necrosis via intracellular signaling.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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