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Merck
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安全性情報

A6982

Sigma-Aldrich

HydroMatrix ペプチド細胞培養スカフォールド

mixture, powder

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About This Item

UNSPSCコード:
12352207

由来生物

synthetic

品質水準

100

形状

powder

保管温度

−20°C

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アプリケーション

HydroMatrix has been shown to support improved growth and migration in the proliferation of many cell types, including neural stem cells, neurons, glia, astrocytes, fibroblasts, and keratinocytes.

構成

HydroMatrix is a peptide nanofiber 3-dimensional scaffold that promotes cell growth and migration. It utilizes specific peptides that self-assemble from fluid precursors into highly crosslinked peptide hydrogels when presented with increased temperatures or ionic strengths. By inducing a rapid transformation from solution to gel, a peptide nanofiber scaffold is generated. Adjusting the concentration of the HydroMatrix solution, researchers can control the flexibility of the 3-D architecture to meet their application′s specific needs.

注意

The lyophilized product at -20°C and is stable for many years at this temperature. The hydrogel should be stored at -70°C to maximize its active span. The 1% (w/v) stock solution should be stored -20°C and is stable for 3-6 months.

調製ノート

This product is supplied as a lyophilized powder. A 1% (w/v) stock solution at 10 mg/mL can be prepared with addition of sterile water, resulting in a rigid gel. A 0.5% (w/v) solution will lead to a softer gel that is more suitable for cell migration and angiogenesis. Lower concentrations in the range of 0.15%-0.25% are more suitable for many cell types including neurons. In aqueous solution, HydroMatrix has a pH of 2.5 and should not be added directly to cells without prior addition of a buffer or medium. Once the hydrogel has formed, do not attempt to mix the gel any further, as this risks destroying the hydrogel integrity. Manipulation of the cells should be carefully performed to avoid disruption of the scaffold.

法的情報

保管分類コード

11 - Combustible Solids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, type N95 (US)

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

A6982-1ML:
A6982-5ML:
A6982-1ML-PW:
A6982-5ML-PW:
A6982-BULK:
A6982-10ML-PW:
A6982-VAR:
A6982-10ML:
A6982-1ML-LBL:
A6982-5ML-LBL:

試験成績書（COA）

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HyStem®-C Cell Culture Scaffold Kit（細胞培養スキャフォールドキット） Trial Kit, For 2.5 mL of hydrogel scaffold solution

Sigma-Aldrich

HYSC010

HyStem®-C Cell Culture Scaffold Kit（細胞培養スキャフォールドキット）

Amish Asthana et al.
Drug discovery today, 17(15-16), 810-817 (2012-04-10)
The three microenvironmental factors that characterize 3D cultures include: first, chemical and/or biochemical composition, second, spatial and temporal dimensions, and third, force and/or substrate physical properties. Although these factors have been studied individually, their interdependence and synergistic interactions have not
Daniela Stoppoloni et al.
Rheumatology international, 33(9), 2399-2403 (2012-03-28)
Osteoarthritis (OA) is one of the most common degenerative joint disease for which there is no cure. It is treated mainly with non-steroidal anti-inflammatory drugs to control the symptoms and some supplements, such as glucosamine and chondroitin sulphate in order
Amish Asthana et al.
Drug discovery today, 18(11-12), 533-540 (2012-12-29)
Force and substrate physical property (pliability) is one of three well established microenvironmental factors (MEFs) that may contribute to the formation of physiologically more relevant constructs (or not) for cell-based high-throughput screening (HTS) in preclinical drug discovery. In 3D cultures
Josine E G Vaes et al.
Glia, 69(3), 655-680 (2020-10-13)
Encephalopathy of prematurity (EoP) is a common cause of long-term neurodevelopmental morbidity in extreme preterm infants. Diffuse white matter injury (dWMI) is currently the most commonly observed form of EoP. Impaired maturation of oligodendrocytes (OLs) is the main underlying pathophysiological

資料

Hydrogels for 3D cell culture

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

3D Cell Culture Tools and Techniques

3D cell culture overview: 3D cell cultures are increasingly being used for basic research and drug discovery. Learn about 2D vs 3D cell culture, advantage of 3D cell culture, and what about the techniques available to develop your 3D cell model.

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