おすすめの製品
由来生物
mouse
品質水準
結合体
unconjugated
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
ARFAG, monoclonal
フォーム
buffered aqueous solution
分子量
antigen 18 kDa
化学種の反応性
bovine, canine, rat, human
濃度
2 mg/mL
テクニック
western blot: 1-2 μg/mL using MDCK total cell extract.
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... ARF6(382)
rat ... Arf6(79121)
詳細
ARF6 is a member of the ADP-ribosylation factor (ARF) family. It is specifically involved in endocytosis of plasma membrane proteins.
Anti-ARF6 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma ARFAG produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide. class III includes only human Arf6. The ADP-ribosylation factors (Arf) family of proteins, which belong to the Ras (rat sarcoma viral oncogene homolog) family, are low molecular weight proteins capable of binding GTP.
アプリケーション
Anti-ARF6 antibody, Mouse monoclonal has been used in:
- western blotting
- enzyme linked immunosorbent assay (ELISA)
- immunocytochemistry
Mouse Monoclonal Anti-ARF6 antibody can be used for western blot assays at 1-2μg/ml using MDCK cell extracts. The antibody can also be used for ELISA and immunocytochemistry.
生物化学的／生理学的作用
ADP-ribosylation factor 6 (Arf6) is present at the plasma membrane and on endosomal membranes where it regulates the flow of trafficking into and out of the cell, and regulates the actin cytoskeleton on the plasma membrane. ARF6 activates phospholipase D (PLD) and phosphatidylinositol 4-phosphate 5-kinase (PIP5K) to generate phosphatidic acid and PIP2 (phosphotidylinositol 4,5-bisphosphate), respectively.
物理的形状
0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A5230-200UL:
A5230-VAR:
A5230-BULK:
Arf6, RalA and BIRC5 protein expression in non small cell lung cancer.
Knizhnik, A.V., et al.
Molecular Biology, 45, 307-315 (2011)
Arf6 regulates the cycling and the readily releasable pool of synaptic vesicles at hippocampal synapse
Tagliatti E, et al.
eLife, 5, e10116-e10116 (2016)
Summer F Acevedo et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(46), 15396-15402 (2015-11-21)
Alcohol use disorders (AUDs) affect people at great individual and societal cost. Individuals at risk for AUDs are sensitive to alcohol's rewarding effects and/or resistant to its aversive and sedating effects. The molecular basis for these traits is poorly understood.
Multiple Roles for Arf6: Sorting, Structuring, and Signaling at the Plasma Membrane
Donaldson JG
The Journal of biological chemistry, 278(43), 41573-41576 (2003)
D A Gonzalez et al.
Molecular psychiatry, 23(3), 621-628 (2017-06-14)
Ubiquitously expressed genes have been implicated in a variety of specific behaviors, including responses to ethanol. However, the mechanisms that confer this behavioral specificity have remained elusive. Previously, we showed that the ubiquitously expressed small GTPase Arf6 is required for
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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