A5170

Anti-Avidin antibody produced in rabbit

whole antiserum

• MDL番号: MFCD00162144
• UNSPSCコード: 12352203
• NACRES: NA.43
• 結合体: unconjugated
• application: ELISA (i); IEP
• クローン: polyclonal
• テクニック: immunoelectrophoresis: suitable; indirect ELISA: 1:100,000
• citations: 7

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: whole antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 含みます: 15 mM sodium azide
• テクニック: immunoelectrophoresis: suitable; indirect ELISA: 1:100,000
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties. Avidin from corn has low molecular weight than chicken egg-derived avidin. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.

免疫原

アビジン

アプリケーション

Anti-Avidin antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA). It has also been used in preparation of antibody-Au nanoparticles.

The presence of Scavidin in BT4C glioma cells was assessed by western blot using a rabbit anti-avidin antibody as the primary at a dilution of 1:4000. Lehtolainen, P. (2002) Cloning and Characterization of Scavidin, a Fusion Protein for the Targeted Delivery of Biotinylated Molecules. J. Biol. Chem. 277:8545-8550.

生物化学的／生理学的作用

Avidin binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.

物理的形状

Supplied as a liquid containing 15mM sodium azide as preservative

調製ノート

treated to remove lipoproteins

アナリシスノート

この抗血清の性能および特異性は、免疫拡散法と免疫電気泳動で評価しています。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable