A2064

抗ヒトIgG−アルカリフォスファターゼ抗体, マウスモノクロナール

clone GG-5, purified from hybridoma cell culture

• 別名: モノクロナール抗ヒトIgG (γ-鎖特異的)
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: alkaline phosphatase conjugate
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: secondary antibodies
• クローン: GG-5, monoclonal
• 形状: buffered aqueous glycerol solution
• 化学種の反応性: human
• テクニック: direct ELISA: 1:50,000; dot blot: 1:80,000; immunohistochemistry: suitable; western blot (chemiluminescent): 1:80,000
• アイソタイプ: IgG1
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

ヒトIgGと結合します。その他のヒトIg分子とは結合しません。抗体はヒトIgGのH鎖上の決定基に特異的です。本抗体は、3% PEG6000存在下の液層で抗原-抗体複合体を形成します。

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.

Monoclonal Anti-Human IgG (Γ-chain specific) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

アプリケーション

Anti-Human IgG- Alkaline Phosphatase antibody, Mouse monoclonal has been used in:

• enzyme linked immunosorbent assay (ELISA)
• western blotting
• dot blot
• immunohistology

Elisa assays were performed using alkaline phosphatase conjugated monoclonal mouse anti-human IgG to detect antibodies against various S. Aureus antigens in the serum from healthy individuals. The antibody was diluted 1:30000 in PBSt and incubated with samples for 2 hours at 37 degrees. Reactin was developed using p-nitrophenyl phosphate substrate (Sigma).

物理的形状

0.05M Trisバッファ-溶液 (pH 8.0, 1mM MgCl₂, 1%BSA, 50%グリセロ-ル, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)