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product name
アクチビンB ヒト, recombinant, expressed in CHO cells, suitable for cell culture
由来生物
human
品質水準
リコンビナント
expressed in CHO cells
アッセイ
≥90% (SDS-PAGE)
形状
lyophilized
有効性
0.30-1.50 ng/mL ED50
分子量
calculated mol wt ~14.5 kDa
包装
pkg of 5 μg
保管条件
avoid repeated freeze/thaw cycles
テクニック
cell culture | mammalian: suitable
不純物
endotoxin, tested
保管温度
−20°C
生物化学的／生理学的作用
アクチビンには、中胚葉誘導、神経細胞分化、骨リモデリング、造血発生、生殖生理学など、広範な生物学的活性があります。アクチビンは、赤血球生成、赤芽球コロニー形成の増強、オキシトシン分泌、パラクリン、自己分泌制御に影響します。
Activin-B is involved in the regulation of a variety of cell functions that overlap the actions of activin-A; however, activin-B also regulates many processes that are distinct from activin-A activities. The functional differences between activin-A and activin-B have recently been reviewed, Thompson TB, et al. (2004).
Similar to activin-A, activin-B modulates follicle stimulating hormone (FSH) secretion and hemoglobin synthesis, DePaolo LV, et al. (1992); Mason AJ, et al. (1989). It is involved in regulation of the menstrual cycle, Liu J, et al. (2001).
Activin-B is not essential for embryo development or survival, but it does have important roles in development. It stimulates spermatogonial proliferation, Mather JP, et al. (1990) and is localized in specific cells, gonocytes and interstitial Leydig cells, and tissues, rete testis and epididymal epithelium, associated with human testis duct system development, Anderson RA, et al. (2002). Activin-B is found in follicle cells surrounding oocytes, Dohrmann CE, et al. (1993) and been shown to increase the rate of oocytes maturation in a dose and time dependent manner, Pang Y and Ge W. (1999, 2002). It is required for successful mammogenesis leading to lactation, Robinson GW and Hennighausen L. (1997). Recent studies suggest that activin-B may regulate adipocyte differentiation, Kogame, M, et al. (2006).
Activin-B has been linked to several aspects of embryo development. Activin-B is first detected in blastula-stage embryos, Thomsen G, et al. (1990) after midblastula transition and homogeneously distributed during blastula and early gastrula stages. It becomes restricted to the dorso-anterior region in neurula-stage embryos and by early tailbud stage it is restricted to brain, eye anlagen, visceral pouches, otic vesicles and the anterior notochord, Dohrmann CE, et al. (1993). Activin-A and -B localizations are different in 3.5 and 4.5 day mouse blastocyst, Paulusman CC, et al. (1994).
Nakamura, T et al. (1992) suggested that the primary function of Activin-B may involve mesoderm-inducing activity and early development modulation. Schrewe H, et al (1994) and Vassalli A, et al. (1994), reported that activin-B was not essential for survival or for mesoderm formation (in mouse), but that it played a role in late fetal development and female fecundity. A role for activin-B in axial formation was suspected because ectopic expression produced second body axis embryos, Thomsen G, et al. (1990), Mitrani E, et al. (1990).
The roles of activin-B during embryo development are starting to emerge. Activin-B may modulate gap junction permeability in embryos, Olson DJ and Moon RT. (1992). Activin-B has been shown to modulate the morphogenesis of the roof plate (RP) of midbrain wherein it inhibits roof plate differentiation, Alexandre P, et al. (2006). Activin-B signals cell cycle arrest in cells of the involuting dorsal axial mesoderm, Ramis JM, et al. (2007).
Activin-B is involved in the development of the adrenal gland and pancreas. It is present in normal adrenal medulla, but absent in the cortex, Salmenkivi K et al. (2001). Interestingly, Activin-B may be a marker for benign adrenal pheochromocytomas, Salmenkivi K et al. (2001).
The role of activin-B in pancreas development is of particular interest because of efforts to use human embryonic stem cells (hESC) as precursors to make insulin producing cells for treatment of diabetes. Activin-B has been shown to promote expression of the pancreas marker Pdx1 gene in cells of differentiated embryoid bodies (EB), in culture, Frandsen U, et al. (2007). Most recently, Jafary H, et al. (2008) induced insulin-secreting cells for ES by adding activin-B to nestin-positive selection protocol cell.
Similar to activin-A, activin-B modulates follicle stimulating hormone (FSH) secretion and hemoglobin synthesis, DePaolo LV, et al. (1992); Mason AJ, et al. (1989). It is involved in regulation of the menstrual cycle, Liu J, et al. (2001).
Activin-B is not essential for embryo development or survival, but it does have important roles in development. It stimulates spermatogonial proliferation, Mather JP, et al. (1990) and is localized in specific cells, gonocytes and interstitial Leydig cells, and tissues, rete testis and epididymal epithelium, associated with human testis duct system development, Anderson RA, et al. (2002). Activin-B is found in follicle cells surrounding oocytes, Dohrmann CE, et al. (1993) and been shown to increase the rate of oocytes maturation in a dose and time dependent manner, Pang Y and Ge W. (1999, 2002). It is required for successful mammogenesis leading to lactation, Robinson GW and Hennighausen L. (1997). Recent studies suggest that activin-B may regulate adipocyte differentiation, Kogame, M, et al. (2006).
Activin-B has been linked to several aspects of embryo development. Activin-B is first detected in blastula-stage embryos, Thomsen G, et al. (1990) after midblastula transition and homogeneously distributed during blastula and early gastrula stages. It becomes restricted to the dorso-anterior region in neurula-stage embryos and by early tailbud stage it is restricted to brain, eye anlagen, visceral pouches, otic vesicles and the anterior notochord, Dohrmann CE, et al. (1993). Activin-A and -B localizations are different in 3.5 and 4.5 day mouse blastocyst, Paulusman CC, et al. (1994).
Nakamura, T et al. (1992) suggested that the primary function of Activin-B may involve mesoderm-inducing activity and early development modulation. Schrewe H, et al (1994) and Vassalli A, et al. (1994), reported that activin-B was not essential for survival or for mesoderm formation (in mouse), but that it played a role in late fetal development and female fecundity. A role for activin-B in axial formation was suspected because ectopic expression produced second body axis embryos, Thomsen G, et al. (1990), Mitrani E, et al. (1990).
The roles of activin-B during embryo development are starting to emerge. Activin-B may modulate gap junction permeability in embryos, Olson DJ and Moon RT. (1992). Activin-B has been shown to modulate the morphogenesis of the roof plate (RP) of midbrain wherein it inhibits roof plate differentiation, Alexandre P, et al. (2006). Activin-B signals cell cycle arrest in cells of the involuting dorsal axial mesoderm, Ramis JM, et al. (2007).
Activin-B is involved in the development of the adrenal gland and pancreas. It is present in normal adrenal medulla, but absent in the cortex, Salmenkivi K et al. (2001). Interestingly, Activin-B may be a marker for benign adrenal pheochromocytomas, Salmenkivi K et al. (2001).
The role of activin-B in pancreas development is of particular interest because of efforts to use human embryonic stem cells (hESC) as precursors to make insulin producing cells for treatment of diabetes. Activin-B has been shown to promote expression of the pancreas marker Pdx1 gene in cells of differentiated embryoid bodies (EB), in culture, Frandsen U, et al. (2007). Most recently, Jafary H, et al. (2008) induced insulin-secreting cells for ES by adding activin-B to nestin-positive selection protocol cell.
物理的形状
凍結乾燥品(30%アセトニトリル、0.1% TFA、0.25 mg BSA含有、0.2 μmフィルター滅菌済み)。
アナリシスノート
生物活性はK562細胞におけるヘモグロビン発現誘導能により測定しています。
シグナルワード
Warning
危険有害性情報
危険有害性の分類
Acute Tox. 4 Inhalation - Eye Irrit. 2
保管分類コード
3 - Flammable liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
dust mask type N95 (US), Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
労働安全衛生法名称等を表示すべき危険物及び有害物
名称等を表示すべき危険物及び有害物
労働安全衛生法名称等を通知すべき危険物及び有害物
名称等を通知すべき危険物及び有害物
Jan Code
A1729-5UG:
A1729-BULK:
A1729-VAR:
A1729-5UG-PW:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Erythroid differentiation bioassays for activin.
R H Schwall et al.
Methods in enzymology, 198, 340-346 (1991-01-01)
A J Mason et al.
Biochemical and biophysical research communications, 135(3), 957-964 (1986-03-28)
The complete amino acid sequences of two forms of human ovarian inhibin have been determined through cloning and nucleotide sequencing of cDNAs encoding their individual subunit precursors. The alpha subunit common to both forms of human inhibin is homologous (84
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