由来生物
rabbit
結合体
unconjugated
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
分子量
antigen ~130 kDa
交差性
rat, mouse
包装
antibody small pack of 25 μL
強化検証
recombinant expression
Learn more about Antibody Enhanced Validation
濃度
1.5 mg/mL
テクニック
western blot: 0.1-0.2 μg/mL using HEK-293 cells expressing mouse Axin-1.
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... AXIN1(8312)
mouse ... Axin1(12005)
rat ... Axin1(79257)
詳細
Axin1 (axis inhibitor-1 or axin) functions as a negative regulator of Wnt signaling. Axin1 is known to associate with several members of the Wnt pathway such as β-catenin, glycogen synthase kinase-3b (GSK-3b), dishevelled (Dsh) and adenomatous polyposis coli (APC),,,.
Rabbit Anti-Axin1 (C-terminal) antibody is specific for rat and mouse axin1 by immunoblotting (∼130 kDa). Staining of the axin1 band in immunoblotting is specifically inhibited by the immunizing peptide
Rabbit Anti-Axin1 (C-terminal) antibody is specific for rat and mouse axin1 by immunoblotting (∼130 kDa). Staining of the axin1 band in immunoblotting is specifically inhibited by the immunizing peptide
免疫原
synthetic peptide corresponding to amino acids 815-832 located near the C-terminal of rat axin1, conjugated to KLH. This sequence is highly conserved (single amino acid substitution) in human, mouse, and bovine axin1.
アプリケーション
Anti-Axin1 (C-terminal) antibody is suitable for use in western blot (0.1-0.2 μg/mL using HEK-293 cells expressing mouse Axin-1). The antibody has also been used for immunoblotting assays in astrocytoma cell lysates.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
物理的形状
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
A0481-25UL:
A0481-VAR:
A0481-BULK:
IXO11584:
A0481-200UL:
最新バージョンのいずれかを選択してください：
Lobna Elkhadragy et al.
Frontiers in oncology, 12, 904031-904031 (2022-06-08)
Hepatocellular carcinoma (HCC) is an aggressive disease lacking effective treatment. Animal models of HCC are necessary for preclinical evaluation of the safety and efficacy of novel therapeutics. Large animal models of HCC allow testing image-guided locoregional therapies, which are widely
Axin induces cell death and reduces cell proliferation in astrocytoma by activating the p53 pathway.
Li-Ying Zhang et al.
International journal of oncology, 35(1), 25-32 (2009-06-11)
Astrocytic tumors are the most common brain tumors with various genetic defects. As a tumor suppressor gene, Axin could control cell death and growth. Axin possesses a separate domain that directly interacts with p53 and regulates the activity of p53
Xiaoli Zhang et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(8), 2790-2795 (2011-08-03)
High expression of the oncoprotein Myc has been linked to poor outcome in human tumors. Although MYC gene amplification and translocations have been observed, this can explain Myc overexpression in only a subset of human tumors. Myc expression is in
S Kishida et al.
The Journal of biological chemistry, 273(18), 10823-10826 (1998-06-06)
The regulators of G protein signaling (RGS) domain of Axin, a negative regulator of the Wnt signaling pathway, made a complex with full-length adenomatous polyposis coli (APC) in COS, 293, and L cells but not with truncated APC in SW480
Thomas J F Nieland et al.
PloS one, 9(3), e91744-e91744 (2014-03-19)
The formation of synapses, the specialized points of chemical communication between neurons, is a highly regulated developmental process fundamental to establishing normal brain circuitry. Perturbations of synapse formation and function causally contribute to human developmental and degenerative neuropsychiatric disorders, such
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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