SBR00028

FITC labeled vancomycin

for fluorescent microbial imaging

• 別名: Vancomycin -FITC, Vancomycin FITC conjugate
• 実験式（ヒル表記法）: C₈₇H₈₈Cl₂N₁₀O₂₉S
• 分子量: 1840.65
• UNSPSCコード: 51102829
• NACRES: NA.76

特性

• 品質水準: 100
• アッセイ: ≥85% (HPLC)
• 形状: solid
• 分子量: M_w 1840.66 g/mol
• 利用可能性: not available in China
• 保管条件: protect from light
• 色: yellow to dark orange
• 適合性: suitable for (Fluorescent detection of gram-positive bacteria); suitable for (Microbiome); suitable for microbiology
• 抗生物質活性スペクトル: Gram-positive bacteria
• 作用機序: DNA synthesis
• 輸送温度: dry ice
• 保管温度: −20°C

詳細

詳細

FITC labeled Vancomycin (Vancomycin-FITC), is a Fluorescein conjugated Vancomycin, with a similar mode of action as Vancomycin. Vancomycin -FITC can be used to detect Gram-positive bacteria by common fluorescent instruments. Vancomycin is a glycopeptide antibiotic, used to treat gram-positive infections. Fluorescent antibiotics are obtained by synthetic conjugation of an antibiotic to a fluorophore.

アプリケーション

Fluorescent antibiotics can be used for many applications including:

• Antimicrobial resistance research.
• Bacterial visualization and imaging.
• Parent antibiotic mode of action research and new antibiotic discovery.
• Toxicity studies.
• Research of bacterial infections and tracking its uptake in vivo.

生物化学的／生理学的作用

Vancomycin inhibits cell wall synthesis at the level of peptidoglycan biosynthesis. Vancomycin binds with high affinity and specificity to the terminal D-Ala-D-Ala of the growing peptide chain during cell wall synthesis. The binding inhibits transpeptidase activity, which prevents further elongation and cross-linking of the peptidoglycan matrix. Vancomycin-FITC has fluorescein attached to the amine group on the disaccharide moiety of the vancomycin molecule. This disaccharide moiety does not play any role in D-Ala-D-Ala binding site, affording a comparable binding affinity of Vancomycin-FITC to Gram-positive bacteria as the parent drug. However, a minimum inhibitory concentration (MIC) decrease of about two orders of magnitude in comparison to Vancomycin was observed. This activity decrease can be linked to repulsive interactions between the negatively charged Fluorescein moiety and the anionic environment of the peptidoglycan.

特徴および利点

• High-quality antibiotic suitable for multiple research applications
• Ideal for immunological, biochemical, and cell biology applications
• Suitable for fluorescent microscopy imaging

アナリシスノート

• Aliquots of the DMSO solution can be stored at -20°C, protected from light for at least 1 month.
• Fluorescent microscopy application: Vancomycin -FITC has excitation/emission wavelength at 495 nm/515 nm.
• It is recommended to dissolve Vancomycin-FITC in DMSO (Dimethyl Sulfoxide) to a concentration of 1mg/mL.
• Recommended working concentration for Bacillus cereus in fluorescent microscopy imaging application is 25μg/mL.

その他情報

For additional information on our range of Biochemicals, please complete this form.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable