PPB006

リン酸緩衝生理食塩水

powder, pH 7.4, pHast Pack^™

• 別名: PBS
• MDL番号: MFCD00131855
• UNSPSCコード: 12161700
• NACRES: NA.21

特性

• product name: リン酸緩衝生理食塩水, pH 7.4, pHast Pack^™, powder
• 製品種目: pHast Pack^™
• 形状: powder
• 保管条件: (Tightly closed. Dry)
• 不純物: <5—2 ppm Heavy metals (Lead)
• 色: white
• pH: 7.4
• 溶解性: soluble, clear, colorless
• 微量陽イオン: Fe: <10 ppm
• その他の活性: DNAse, none detected; Nickase, none detected; Protease, none detected; RNAse, none detected
• 保管温度: room temp

詳細

詳細

Phosphate buffered saline or PBS, pH 7.4 is a commonly used biological buffer in molecular biology. A 1X working solution is typically used in cell culture and electrophoresis assays to wash away non-adherent cells or any unbound biomolecules. PBS is hypo-osmotic and can be harmful to cells, causing swelling and resulting in cell lysis, if left for extended periods.

アプリケーション

Phosphate buffered saline is commonly used in washing solutions for:

• ELISA
• Western Blotting and other protein applications
• Cell culture (sterile filtration recommended)
• Resuspension and sonication of cells
• Immunohistochemistry

特徴および利点

• Save time with our ready-to-use formulation
• No calculations, measurements, and adjusting pH
• Reliable quality for reproducibility
• Suitable for use in cell culture and electrophoresis
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

包装

Foil pouches

再構成

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 10mM Na2HPO4, 1.8mM KH2PO4, 138mM NaCl, 2.7mM KCl, pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

保管および安定性

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

その他情報

For additional information on our range of Biochemicals, please complete this form.

法的情報

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable