おすすめの製品
品質水準
使用法
sufficient for 100 reactions
sufficient for 1000 reactions
sufficient for 500 reactions
sufficient for 5000 reactions
シェルフライフ
≤12 mo.
特徴
dNTPs included
hotstart
包装
kit of 1 mL (100 x 20 μL rxn; KK4701)
kit of 10 mL (1000 x 20 μL rxn; KK4703)
kit of 5 mL (500 x 20 μL rxn; KK4702)
kit of 50 mL (5000 x 20 μL rxn; KK4715)
メーカー／製品名
Roche
濃度
2 ×
テクニック
qPCR: suitable
入力
purified DNA
適合性
for use with Agilent Mx3000P
for use with Agilent Mx3005P
for use with Agilent Mx4000
for use with Applied Biosystems® 5700
for use with Applied Biosystems® 7000
for use with Applied Biosystems® 7300
for use with Applied Biosystems® 7500
for use with Applied Biosystems® 7700
for use with Applied Biosystems® 7900HT
for use with Applied Biosystems® ViiA 7
for use with QuantStudio™
for use with StepOne™
for use with StepOnePlus™
検出方法
probe-based
保管温度
−20°C
類似した製品をお探しですか？ 訪問 製品比較ガイド
詳細
KAPA PROBE FAST qPCR Kits provide fast and reproducible results for all probe-based qPCR applications. Kits contain a ready-to-use master mix for highly sensitive and accurate real-time PCR using sequence-specific fluorogenic probe chemistries, including hydrolysis probes (e.g. TaqMan®), FRET probes, and displacement probes (e.g. molecular beacons).
アプリケーション
KAPA PROBE FAST may be used for:
- Real-time quantitative methylation-specific PCR (qMSP)
- Genotyping
- Gene expression analysis
- Multiplex qPCR and qRT-PCR
- SNP genotyping/allelic discrimination
- Microarray validation
特徴および利点
Accurately discriminate between alleles:
Achieve excellent reproducibility and efficiency:
Increase flexibility:
Quick Notes:
- Exceptional differentiation of heterozygous and homozygous alleles
- Discrete clusters in SNP genotyping assays
Achieve excellent reproducibility and efficiency:
- Fast, high performance five-colored multiplex PCR
- Similar abundance levels achieved with low copy number
- Broad dynamic range of up to 10 orders of magnitude
Increase flexibility:
- Compatible with both standard and fast cycling protocolsRetain accurate quantification
Quick Notes:
- These kits are compatible with all fluorogenic probebased technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan®) and displacement probes (e.g., molecular beacons).
- Initial denaturation for 20 sec at 95ºC is sufficient for enzyme reactivation; however, optimal denaturation of complex targets may require up to 3 min denaturation.
- For 2-step cycling, use 30 sec combined annealing/ extension/data acquisition.
- For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition.
- Do not exceed 25 μL volumes.
品質
KAPA PROBE FAST qPCR Master Mix (2X) is subjected to stringent quality control tests, is free of contaminating exoand endonuclease activity, and meets strict requirements with respect to DNA contamination levels.
調製ノート
ROX/fluorescein reference dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity. Avoid repeated freezing and thawing. Always ensure that the product has been fully thawed and mixed before use.
KAPA PROBE FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week, and may be stored at this temperature for short-term use provided that they do not become contaminated with microbes and/or nucleases.
KAPA PROBE FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week, and may be stored at this temperature for short-term use provided that they do not become contaminated with microbes and/or nucleases.
その他情報
For Research Use Only. Not for use in diagnostic procedures.
法的情報
Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
QuantStudio is a trademark of IROA Technologies LLC
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
キットの構成要素のみ
製品番号
詳細
- KAPA Taq HotStart® DNA Polymerase
- 50X ROX™ High Reference Dye
- 50X ROX™ Low Reference Dye
- reaction buffer
- dNTPs
- MgCl2 5 mM
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
does not flash
引火点(℃)
does not flash
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Cell-free methylation markers with diagnostic and prognostic potential in hepatocellular carcinoma.
Lu C Y, et al.
Oncotarget, 8(4), 6406-6406 (2017)
James E Ussher et al.
Medicine, 94(29), e1134-e1134 (2015-07-23)
Mucosal-associated invariant T (MAIT) cells are an abundant antibacterial innate-like lymphocyte population. There are conflicting reports as to their fate in HIV infection. The objective of this study was to determine whether MAIT cells are truly depleted in HIV infection.
Long non-coding RNA MEG3 functions as a competing endogenous RNA to regulate gastric cancer progression.
Peng W, et al.
Journal of Experimental & Clinical Cancer Research, 34(1), 79-79 (2015)
Andreas A Argyriou et al.
Cancer, 119(19), 3570-3577 (2013-07-04)
The current prospective, multicenter study sought to identify single nucleotide polymorphisms of voltage-gated sodium channels (SCNAs) genes that might confer susceptibility to an increased incidence and severity of oxaliplatin-induced peripheral neuropathy (OXAIPN) in patients treated with either leucovorin, 5-fluorouracil, and
Holger Linck et al.
PloS one, 12(5), e0177808-e0177808 (2017-05-26)
Rubus stunt is an economically important disease in the production of raspberries, blackberries, and loganberries. A fast, sensitive, and reliable diagnosis of phytoplasmas, the causal agent of the disease, is of prime importance to stop its spread by vegetative propagation
資料
An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
製品に関するお問い合わせはこちら（テクニカルサービス）