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Merck
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主要文書

安全性情報

UC0E03

Sigma-Aldrich

UCOE® Dual Expression Puromycin Vector Set

別名:

UCOE Mammalian Protein Expression Vectors, UCOE Mammalian Protein Expression Plasmids, Ubiquitous Chromatin Opening Element, UCOE03

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About This Item

UNSPSCコード:
41106621
eCl@ss:
34360190
NACRES:
NA.51

輸送温度

dry ice

品質水準

100

詳細

The Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing.

CET 1019 HD-puro-SceI is a mammalian expression plasmid vector containing both the mouse Rps3 UCOE and the human HNRPA2B1-CBX3 UCOE each upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element.

CET 1019 AD-puro-SceI is a mammalian expression plasmid vector containing both the mouse Rps3 UCOE and the human HNRPA2B1-CBX3 UCOE each upstream of a guinea pig CMV (gpCMV) promoter-enhancer element.

The CET 1019 HD-puro-SceI and CET 1019 AD-puro-SceI vectors possess the following features:

  • A multiple cloning site downstream of the UCOE-CMV combinations containing unique restriction enzyme sites to permit easy insertion of any gene of interest (cDNA or genomic)
  • A polyadenylation element from the SV40 early region located downstream of the multiple cloning site for appropriate mRNA 3 end formation
  • A puromycin antibiotic resistance gene for selection of stably transfected mammalian cells
  • A β-lactamase gene for plasmid selection and propagation in transformed prokaryotic cells in the presence of ampicillin
  • An I-SceI homing restriction endonuclease site for linearization of constructs prior to transfection into mammalian cells, which favors integration into the target cell genome via the pre-generated free DNA ends and thus retains the integrity of the UCOE-CMV-transgene cassettes


THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT licensing@emdmillipore.com.

構成

1) 10ug CET 1019 HD-puro-SceI (+) Vector (CS221292)

2) 10ug CET 1019 AD-puro-SceI (+) Vector (CS221293))

3) 10ug DC HD-puro (-) Vector (CS221283)

4) 10ug DC AD-puro (-) Vector (CS221295)

その他情報

Concentration: Please refer to lot specific datasheet.

法的情報

UCOE is a registered trademark of Merck KGaA, Darmstadt, Germany

保管分類コード

12 - Non Combustible Liquids

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

UC0E03:

試験成績書（COA）

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以前この製品を購入いただいたことがある場合

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Michael Antoniou et al.
Genomics, 82(3), 269-279 (2003-08-09)
The genetic elements that are responsible for establishing a transcriptionally competent, open chromatin structure at a region of the genome that consists only of ubiquitously expressed, housekeeping genes are currently unknown. We demonstrate for the first time through functional analysis

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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