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Key Documents

SCT086

Sigma-Aldrich

BioTracker MitoBlue Live Cell Dye

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About This Item

UNSPSCコード:
12352207

由来生物

Unknown

包装

vial of 1 mg

メーカー/製品名

Millipore

成長モード

N/A

テクニック

cell culture | mammalian: suitable

輸送温度

dry ice

保管温度

−20°C

詳細

BioTracker MitoBlue Live Cell Dye is a synthetic bisbenzamidine derivative that selectively stains functional mitochondria while displaying low toxicity, bright blue emission, and high resistance to photobleaching in both live and fixed cells (1). It is a unique mitochondrial dye in that after initially staining mitochondria, the dye is transported to lysosomes in a time-dependent manner. This distinct staining pattern allows for monitoring and tracking the mitochondrial recycling mechanism in living cells (2). MitoBlue has been shown to be suitable for two-photon microscopy using a 740 nm two-photon excitation (TPE) wavelength and emission channel at 480 nm (2).

Spectral Properties</bold>
Absorbance: 329nm
Emission: 420nm

Reference:
1. Sa?nchez, Mateo I., et al. "MitoBlue: a nontoxic and photostable blue-emitting dye that selectively labels functional mitochondria." ACS chemical biology 9.12 (2014): 2742-2747.
2. Sánchez, Mateo I., et al. "MitoBlue as a tool to analyze the mitochondria-lysosome communication." Scientific reports 10.1 (2020): 1-12.

アプリケーション

Purity >97% confirmed by HPLC. Identification confirmed by HNMR, LC-MS, and elemental analysis.

特徴および利点

Live cell imaging dye that stains functional mitochondria and tracks the mitochondrial degradation process and transport to lysosomes.

物理的形状

Lyophilized. Yellow solid.

保管および安定性

Store at -20°C, desiccate and protect from light.Note: Centrifuge vial briefly to collect contents at bottom of vial before opening

その他情報

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

ピクトグラム

Exclamation mark

シグナルワード

Warning

危険有害性情報

危険有害性の分類

Eye Irrit. 2 - Skin Irrit. 2

保管分類コード

11 - Combustible Solids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable


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