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包装
vial of ≥1X10⁶ cells
テクニック
cell culture | stem cell: suitable
輸送温度
dry ice
保管温度
−196°C
詳細
The discovery that somatic cells could revert back to pluripotent like cells in 2006 by Shinya Yamanaka created an entirely new area of stem cell biology. Human iPS cells removed the ethical concerns associated with embryonic stem cells and allowed scientists to model human diseases that were previously impossible to model such as Alzheimer′s, Parkinson′s and Autism. Human induced pluripotent stem cells (iPSCs), have the capacity to give rise to differentiated progeny representative of all three germ layers (ectoderm, endoderm, and mesoderm). The ability to expand pluripotent cells in vitro and subject them to direct differentiation to produce specific cell types is crucial for the development of potential cell-based therapies and disease-in-a-dish cell models for research.
Simplicon® RNA Reprogramming Technology is a next generation non-integrating reprogramming system that uses a single synthetic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells. The single RNA strand contains the four reprogramming factors, OCT-4, KLF-4, SOX-2 and GLIS1 (OKSG) and enables extremely efficient reprogramming using a single transfection step without any viral intermediates or host genome integration. TheSimplicon® human iPS cell line was generated by reprogramming human foreskin fibrobalsts (SCC058) using theSimplicon® RNA Reprogramming Kit (SCR550). The cell line is integration-free, highly characterized and maintains a pluripotent phenotype over multiple passages.
Simplicon® RNA Reprogramming Technology is a next generation non-integrating reprogramming system that uses a single synthetic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells. The single RNA strand contains the four reprogramming factors, OCT-4, KLF-4, SOX-2 and GLIS1 (OKSG) and enables extremely efficient reprogramming using a single transfection step without any viral intermediates or host genome integration. TheSimplicon® human iPS cell line was generated by reprogramming human foreskin fibrobalsts (SCC058) using theSimplicon® RNA Reprogramming Kit (SCR550). The cell line is integration-free, highly characterized and maintains a pluripotent phenotype over multiple passages.
アプリケーション
Highly characterized integration-free human induced pluripotent stem cell (iPSC) line generated using Simplicon® RNA reprogramming technology.
包装
≥1X106 cells/vial
>=1X106 cells/vial
保管および安定性
Simplicon® human iPS cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
その他情報
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
法的情報
SIMPLICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
劇物
労働安全衛生法名称等を表示すべき危険物及び有害物
名称等を表示すべき危険物及び有害物
労働安全衛生法名称等を通知すべき危険物及び有害物
名称等を通知すべき危険物及び有害物
Jan Code
SCC271:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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