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Merck

RADPKMAG-80K

Millipore

MILLIPLEX® Rat Adipokine Panel - Metabolism Assay

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About This Item

UNSPSCコード:
12161503
eCl@ss:
32161000
NACRES:
NA.84

化学種の反応性

rat

品質水準

メーカー/製品名

Milliplex®

assay range

accuracy: 94-119%
sensitivity: 0.3-17 pg/mL
(MinDC)

standard curve range: 0.7-3,000 pg/mL
(IL-1β, TNFα)

standard curve range: 12-50,000 pg/mL
(PAI-1)

standard curve range: 15-60,000 pg/mL
(IL-6, Leptin)

standard curve range: 24-100,000 pg/mL
(Insulin)

standard curve range: 9.8-40,000 pg/mL
(MCP-1)

テクニック

multiplexing: suitable

検出方法

fluorometric (Luminex xMAP)

輸送温度

wet ice

詳細

In the past, the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism.

MILLIPLEX® Rat Adipokine Magnetic Bead Panel is a seven-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum and plasma samples: IL-1β, IL-6, Insulin, Leptin, MCP-1, PAI-1, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism
The MILLIPLEX® MAP Rat Adipokine Magnetic Bead Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: IL-1β, IL-6, Insulin, Leptin, MCP-1, PAI-1 Total, and TNFα.

特異性

Cross Reactivty
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

アプリケーション

  • Analytes: IL-1β, IL-6, Insulin, Leptin, MCP-1 (CCL2), PAI-1 (Total), TNFα
  • Recommended Sample Type: Rat serum or plasma
  • Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma
  • Assay Run Time: Overnight (16-18) hours at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Endocrine; Obesity; Metabolic Disease

特徴および利点

Design your multiplex kit by choosing available analytes within this panel.

包装

Everything you need in a single kit.

構成

Rat Adipokine Standard, 1 vial, lyophilized

Rat Adipokine Quality Control 1, 1 vial, lyophilized

Rat Adipokine Quality Control 2, 1 vial, lyophilized

Assay Buffer, 1 bottle, 30 mL

Bead Diluent, 1 vial, 3.5 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Rat Adipokine Detection Antibodies, 1 bottle, 5.5 mL

Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

保管および安定性

Recommended storage for kit components is 2 - 8°C.

その他情報

Please contact Technical Service for linearity of dilution.
Sensitivity: Please see kit protocol for individual assay sensitivities.

法的情報

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

ピクトグラム

Skull and crossbonesEnvironment

シグナルワード

Danger

危険有害性情報

危険有害性の分類

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

保管分類コード

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

RADPKMAG-80K-01:
RADPKMAG-80K-06:
RADPKMAG-80K-03:
RADPKMAG-80K-07:
RADPKMAG-80K-05:
RADPKMAG-80K-04:
RADPKMAG-80K:
RADPKMAG-80K-02:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Jaroslava Trnovska et al.
International journal of molecular sciences, 22(19) (2021-10-14)
(1) Background: empagliflozin, sodium-glucose co-transporter 2 (SGLT-2) inhibitor, is an effective antidiabetic agent with strong cardio- and nephroprotective properties. The mechanisms behind its cardio- and nephroprotection are still not fully clarified. (2) Methods: we used male hereditary hypertriglyceridemic (hHTG) rats
Lin Song et al.
Biology of reproduction, 102(3), 705-716 (2019-11-20)
Maternal high-fat (HF) diet negatively affects maternal metabolism and placental function. This study aimed to determine whether gestational exercise prevents the effect of HF diet on placental amino acid transporter expression and nutrient-sensing signaling and the fetal response. Pregnant Sprague-Dawley

関連コンテンツ

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

実験動物、コンパニオンアニマル、農業用動物の研究におけるバイオマーカー検出マルチプレックス・イムノアッセイの利点と、獣医学および動物衛生研究のためのMILLIPLEX®マルチプレックスアッセイについて説明します。

MILLIPLEX®マルチプレックス代謝アッセイなどのマルチプレックス・イムノアッセイは、メタボリックシンドローム研究に重要です。糖尿病や肥満など、関連するさまざまな病態の全体像を把握でき、時間とサンプル量を節約できるためです。

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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