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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
4B11, monoclonal
形状
liquid
含まれません
preservative
化学種の反応性
mouse, human
メーカー／製品名
Calbiochem®
保管条件
OK to freeze
avoid repeated freeze/thaw cycles
アイソタイプ
IgG2a
輸送温度
wet ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... MDM2(4193)
mouse ... Mdm2(17246)
詳細
Purified mouse monoclonal antibody. Recognizes the ~90 kDa (apparent MW) MDM2 protein.
Recognizes the ~90 kDa (apparent MW) MDM2 protein in A549 cells. May also recognize the ~60 kDa and ~90 kDa isoforms of MDM2.
This Anti-MDM2 (Ab-3) Mouse mAb (4B11) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MDM2 (Ab-3).
免疫原
Epitope: within amino acids 383-491
Human
full-length, recombinant human MDM2
アプリケーション
Immunoblotting (2 g/ml, chemiluminescence)
Immunofluorescence (1 g/ml)
Immunoprecipitation (1 g/reaction)
Paraffin Sections (2.5 g/ml, heat pre-treatment required)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Standard Handling (A)
物理的形状
In 50 mM sodium phosphate buffer, 50% glycerol.
再構成
Following initial thaw, aliquot and freeze (-20°C).
アナリシスノート
Positive Control
A549 cells
A549 cells
その他情報
Marchetti, A., et al. 1995. J. Pathol.175, 31.
Barak, Y., et al. 1993. EMBO. J.12, 461.
Ladanyi, M., et al. 1993. Cancer Res.53, 16.
Leach, F. S., et al. 1993. Cancer Res.53, 2231.
Oliner, J. D., et al. 1993. Nature362, 857.
Momand, J., et al. 1992. Cell69, 1237.
Oliner, J. D., et al. 1992. Nature358, 80.
Fakharzadeh, S. S., et al. 1991. EMBO. J.10, 1565.
Barak, Y., et al. 1993. EMBO. J.12, 461.
Ladanyi, M., et al. 1993. Cancer Res.53, 16.
Leach, F. S., et al. 1993. Cancer Res.53, 2231.
Oliner, J. D., et al. 1993. Nature362, 857.
Momand, J., et al. 1992. Cell69, 1237.
Oliner, J. D., et al. 1992. Nature358, 80.
Fakharzadeh, S. S., et al. 1991. EMBO. J.10, 1565.
May also recognize the ~60 kDa and ~90 kDa isoforms of MDM2. Antibody should be titrated for optimal results in individual systems.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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保管分類コード
10 - Combustible liquids
WGK
WGK 3
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
OP143-100UG:
OP143-ML:
OP143-MG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
F S Leach et al.
Cancer research, 53(10 Suppl), 2231-2234 (1993-05-15)
The p53 and MDM2 genes were analyzed in 24 human soft tissue sarcomas (11 malignant fibrous histiocytomas and 13 liposarcomas). Alterations of p53, consisting of point mutations, deletions, or overexpression, were detected in one-third (8 of 24) of the sarcomas.
M Ladanyi et al.
Cancer research, 53(1), 16-18 (1993-01-01)
The human homologue of the murine double minute 2 gene (MDM2), a p53-binding protein which may act as a regulator of p53 protein function, has recently been cloned. Initial studies of this gene in a variety of human tumors have
J Momand et al.
Cell, 69(7), 1237-1245 (1992-06-26)
A cellular phosphoprotein with an apparent molecular mass of 90 kd (p90) that forms a complex with both mutant and wild-type p53 protein has been characterized, purified, and identified. The protein was identified as a product of the murine double
A Marchetti et al.
The Journal of pathology, 175(1), 31-38 (1995-01-01)
This study investigates the mdm2 gene status and expression in 66 surgically resected human breast carcinomas, with correlations with clinico-pathological and biological data (histological type, grading, steroid receptor status, p53 expression, proliferative activity). Four (7.7 per cent) out of 52
J D Oliner et al.
Nature, 362(6423), 857-860 (1993-04-29)
The tumour-suppressor gene p53 is inactivated in most human malignancies either by missense mutations or by binding to oncogenic proteins. In human soft tissue sarcomas, inactivation apparently results from MDM2 gene amplification. MDM2 is an oncogene product that may function
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