MABT1503

Anti-PGM5 (Aciculin) Antibody, clone 14F8

clone 14F8, from mouse

• 別名: Aciculin, Phosphoglucomutase-like Protein 5, PGM-RP
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 14F8, monoclonal
• 化学種の反応性: human, rabbit, rat, bovine, mouse
• 化学種の反応性（ホモロジーによる予測）: frog, avian, porcine
• 包装: antibody small pack of 25 μg
• テクニック: ELISA: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_068800
• UniProtアクセッション番号: Q15124
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PGM5(5239)

詳細

詳細

Phosphoglucomutase-like protein 5 (UniProt: Q15124; also known as Aciculin, Phosphoglucomutase-related protein, PGM-RP) is encoded by the PGM5 (also known as PGMRP) gene (Gene ID: 5239) in human. Aciculin is a dystrophin and utrophin-binding protein that is highly homologous to the glycolytic enzyme phosphoglucomutase type 1. However, it lacks phosphoglucomutase activity. Aciculin is a component of adherens-type cell-cell and cell-matrix junctions and is concentrated in focal contacts at the ends of actin bundles and associates with actin filaments. Aciculin is also reported to be an interaction partner of Filamin C and Xin proteins. Higher levels of Aciculin are reported in smooth and cardiac muscle and lower levels are present in skeletal muscle, liver, kidney and brain tissue. In striated muscle, it mainly localizes to the intercalated discs of the heart and to myotendinous junctions. Low levels of Aciculin are expressed in 10-12-week-old fetus and these levels increase progressively into infancy reaching maximum levels at maturity. Two isoforms of Aciculin have been reported that are produced by alternative splicing. Aciculin knockdown in myotubes is shown to cause failure in myofibril assembly, alignment and membrane attachment, and a massive reduction in myofibril number. (Ref.: Molt, S., et al., (2014). J. Cell Sci.127(16); 3578-3592).

特異性

Clone 14F8 detects Phosphoglucomutase-like protein 5 in mouse and rat cardiac tissue and is predicted to react with human.

免疫原

Cytoskeletal fraction of the human myometrium tissue extract.

アプリケーション

Research Category
細胞骨格

Anti-PGM5 (Aciculin), clone 14F8, Cat. No. MABT1503, is a mouse monoclonal antibody that detects Phosphoglucomutase-like protein 5 and has been tested for use in ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Western Blotting Analysis: 1 µg/mL from a representative lot detected PGM5 (Aciculin) in rat heart tissue lysate.

Immunoprecipitation Analysis: A representative lot immunoprecipitated PGM5 (Aciculin) in Immunoprecipitation applications (Belkin, A.M., et. al. (1995). J Biol Chem. 270(11):6328-37; Belkin, A.M., et. al. (1995). Exp Cell Res. 221(1):132-40).

Immunofluorescence Analysis: A representative lot detected PGM5 (Aciculin) in Immunofluorescence applications (Belkin, A.M., et. al. (1994). J Cell Sci. 107 ( Pt 7):1993-2003).

Immunohistochemistry Analysis: A representative lot detected PGM5 (Aciculin) in Immunohistochemistry applications (Belkin, A.M., et. al. (1995). Exp Cell Res. 221(1):132-40).

Western Blotting Analysis: A representative lot detected PGM5 (Aciculin) in Western Blotting applications (Belkin, A.M., et. al. (1995). J Biol Chem. 270(11):6328-37; Belkin, A.M., et. al. (1994). J Cell Sci. 107 ( Pt 7):1993-2003).

ELISA Analysis: A representative lot detected PGM5 (Aciculin) in ELISA applications (Molt, S., et. al. (2014). J Cell Sci. 127(Pt 16):3578-92).

品質

Evaluated by Western Blotting in mouse heart tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected PGM5 (Aciculin) in mouse heart tissue lysate.

ターゲットの説明

~57 kDa observed; 62.22 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1