MABT1487

Anti-E-Cadherin/CD324 Antibody, clone 19A11

• 別名: CAM 120/80, CD324, Cadherin-1, Epithelial cadherin, Uvomorulin
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: mouse
• 品質水準: 200
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 19A11, monoclonal
• 分子量: calculated mol wt 97 kDa
• 精製方法: using protein G
• 交差性: human, canine
• 包装: antibody small pack of 100
• テクニック: ELISA: suitable; immunocytochemistry: suitable; inhibition assay: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• エピトープ配列: N-terminal extracellular domain
• タンパク質IDアクセッション番号: NP_004351
• UniProtアクセッション番号: P12830
• 保管温度: -10 to -25°C
• 遺伝子情報: human ... CDH1(999)

詳細

特異性

Clone 19A11 is a mouse monoclonal antibody that detects E-Cadherin/CD324. It targets an epitope within the extracellular domain.

免疫原

Recombinant protein corresponding to the extracellular domain of human E-Cadherin.

アプリケーション

Quality Control Testing

Evaluated by Immunocytochemistry in MCF-7 cells.

Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected E-Cadherin/CD324 in MCF-7 cells.

Tested Applications

Agonist Function:: A representative lot stimulated adhesion of cells to pure E-Cadherin substrate (Petrova, Y., et al. (2012). Mol Biol Cell.;23(11):2092-108).

Western Blotting Analysis: A representative lot detected E-Cadherin/CD324) in Western Blotting applications (Petrova, Y., et al. (2012). Mol Biol Cell. 23(11):2092-108; Xie, B., et al. (2022). Proc Natl Acad Sci USA. 119(32):e2204473119).

Immunocytochemistry Analysis: A representative lot detected E-Cadherin/CD324 in Immunocytochemistry applications (Petrova, Y., et al. (2012). Mol Biol Cell. 23(11):2092-108).

ELISA Analysis: A representative lot detected E-Cadherin/CD324 in ELISA applications (Petrova, Y., et al. (2012). Mol Biol Cell. 23(11):2092-108).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

Cadherin-1 (UniProt: P12830; also known as CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324) is encoded by the CDH1 (also known as CDHE, UVO) gene (Gene ID: 999) in human. Cadherins are calcium-dependent cell adhesion molecules that participate in cell-cell adhesion during embryogenesis, development, organogenesis, and differentiation. E-cadherin is a single-pass type I membrane glycoprotein with tumor suppressive properties. It is synthesized with a signal peptide (aa 1-22) and a propeptide (aa 23-154) that are subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 155-709), a transmembrane domain (aa 710-730), and a cytoplasmic domain (aa 731-882). N-glycosylation at Asn-637 is shown to be essential for its expression, folding, and trafficking. E-cadherin is known to contain five cadherin domains. Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain. Post-translationally it can be cleaved into three chains: E-Cad/CTF1 (aa 701-882); E-Cad/CTF2 (aa 732-882); and E-Cad/CTF3 (aa 751-882). During apoptosis or with calcium influx, it is cleaved by a membrane-bound metalloproteinase (ADAM10; at residues 700-701), which causes disruption of cell-cell adhesion and the subsequent release of β-catenin into the cytoplasm. The residual membrane-tethered cleavage product is then rapidly degraded via an intracellular proteolytic pathway. It can also be cleaved by PS1/ -secretase (at residues 731-732) and this cleavage promotes disassembly of adherens junctions. Clone 19A11 is an activating monoclonal antibody that stimulates adhesion of cells to E-cadherin substrate. It modifies the adhesive properties of E-cadherin and prevent the metastatic invasion of mouse lung cancer cells expressing human E-cadherin. This clone is shown to bind to the extracellular domain of E-cadherin near its primary adhesive motif: the strand swap dimer interface, which strengthens adhesion by stabilizing strand-swap dimers. (Ref.: Xie, B., et al. (2022). Proc. Natl. Acad. Sci. USA. 119 (32); e2204473119; Petrova, YI., et al. (2012). Mol. Biol. Cell. 23(11); 2092-2108; Marambaud, M., et al. (2002). EMBO J. 21(8); 1948-1956; Steinhausen, U., et al. (2001). J. Biol. Chem. 276(7); 4972-4980).

物理的形状

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

再構成

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable