MABT1460

Anti-LAMC2 Antibody

• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: P2H, monoclonal
• 分子量: calculated mol wt 130.98 kDa
• 化学種の反応性: mouse, human
• 包装: antibody small pack of 100 μL
• テクニック: ELISA: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• アイソタイプ: IgG2a
• UniProtアクセッション番号: Q13753
• 輸送温度: dry ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... LAMC2(3918)

詳細

詳細

Laminin subunit g2 (UniProt: Q13753; also known as Cell-scattering factor 140 kDa subunit, CSF 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma) is encoded by the LAMC2 (also known as LAMB2T, LAMNB2) gene (Gene ID: 3918) in human. Laminin is a major basement membrane glycoprotein consisting of α, β, γ chains that are bound to each other by disulfide bonds. Over 15 different isoforms have been reported in the laminin family that are expressed in a tissue-specific manner and play different role in each tissue. γ², a subunit of Laminin-5 is reported to be frequently over-expressed as a monomer or as the β²−γ³ heterodimer in invasive cancers and is regarded as an invasion marker. It has 8 EGF-like domains. The cell adhesion and motility activities of Laminin-5 are reported to be regulated by the proteolytic cleavage of the short arm of Laminin γ² chain by BMP-1 or MT1-MMP. This cleavage releases a 45 kDa N-terminal proteolytic fragment (γ²pf). The short arm and γ²pf can be further cleaved by serine proteases and by matrix metalloproteinases resulting in the release of small N-terminal fragments. γ²pf-like fragments have been reported to be present in conditioned media of cultured cancer cells and in sera from human cancer subjects. Clone P2H targets the second N-terminal EGF-like repeat (NE2) of domain V of laminin 2. (Ref.: Sato, H., et al. (2014). Cancer Sci. 105(2); 168-175; Miyazaki, K., et al. (2016). Cancer Sci. 107(12); 1909-1918).

特異性

Clone P2H is a mouse monoclonal antibody that detects Laminin-5, g2 chain. It targets an epitope within the N-terminal, NE2 in domain V.

免疫原

His-tagged 45-kDa N-terminal fragment consisting of g2pf, domain V.

アプリケーション

Quality Control Testing

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Laminin-5 subunit g2 (LAMC2) in A431 cells.

Tested Applications

Western Blotting Analysis: 1 μg/mL from a representative lot detected LAMC2 in lung squamous cell carcinoma cell lysates (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

Western Blotting Analysis: A representative lot detected LAMC2 in Western Blotting applications (Miyazaki, K., et. al. (2016). Cancer Sci. 107(12):1909-1918).

Immunohistochemistry (Paraffin) Analysis: 10 μg/mL from a representative lot detected LAMC2 in normal human skin, skin cancer, normal breast, and breast adenocarcinoma tissues (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

ELISA Analysis: 5 μg/mL from a representative lot detected LAMC2 in Lm-gamma2pf and Lm-gamma2 in Lm332 condition medium from Lm332-expressing HEK293 cells (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

Immunocytochemistry Analysis: A representative lot detected LAMC2 in Immunocytochemistry applications (Miyazaki, K., et. al. (2016). Cancer Sci. 107(12):1909-1918).

Immunohistochemistry Applications: A representative lot detected LAMC2 in Immunohistochemistry applications of lung squamous cell carcinomas and lung adenocarcinomas (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-LAMC2, clone P2H, Cat. No. MABT1460, is a mouse monoclonal antibody that detects Laminin subunit g2 and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

物理的形状

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at +2°C to +8°C from date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable