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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
7H4.2, monoclonal
化学種の反応性
mouse, human
包装
antibody small pack of 25 μg
テクニック
immunohistochemistry: suitable (paraffin)
western blot: suitable
アイソタイプ
IgG2bκ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
ambient
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... ACACA(31)
mouse ... Acaca(107476)
詳細
Acetyl-CoA carboxylase 1 (UniProt: Q13085; also known as ACC1, ACC-alpha) is encoded by the ACACA (also known as ACAC, ACC1, ACCA) gene (Gene ID: 31) in human. It catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. ACC1 is a multi-subunit, biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. Two isoforms fof ACC have been reported, alpha and beta that are encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. ACC1 levels are regulated under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. ACC1 activity is inhibited upon phosphorylation, which occurs when glucagon or epinephrine binds to the receptors or when the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acids inhibits the activities of ACC1. ACC is considered as an important target in the design of new anti-obesity drugs.
特異性
Clone 7H4.2 detects acetyl-CoA carboxylase 1 in human. It targets an epitope within 86 amino acids from the C-terminal end.
免疫原
GST/His-tagged recombinant fragment corresponding to 86 amino acids from the C-terminal end of human Acetyl-CoA carboxylase 1.
アプリケーション
Anti-Acetyl CoA Carboxylase 1 Antibody, clone 7H4.2, Cat. No. MABS830, is a mouse monoclonal antibody that targets Acetyl CoA Carboxylase 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Acetyl CoA Carboxylase 1 in human tonsil, human testis, and human cervical cancer tissue.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Acetyl CoA Carboxylase 1 in 10 µg of NIH/3T3 cell lyate.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Acetyl CoA Carboxylase 1 in 10 µg of NIH/3T3 cell lyate.
品質
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Acetyl CoA Carboxylase 1 in 10 µg of A431 cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Acetyl CoA Carboxylase 1 in 10 µg of A431 cell lysate.
ターゲットの説明
~265 kDa observed; 265.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
物理的形状
Format: Purified
その他情報
Concentration: Please refer to lot specific datasheet.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABS830:
MABS830-25UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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