MABS415

Anti-PPM1A, clone 7F12 Antibody

clone 7F12, from mouse

• 別名: PP2C-alpha, Protein phosphatase IA, Protein phosphatase 2C isoform alpha, PP2CA, PPM1A, PPPM1A
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 7F12, monoclonal
• 化学種の反応性: human
• テクニック: ELISA: suitable; flow cytometry: suitable; western blot: suitable
• アイソタイプ: IgG1
• UniProtアクセッション番号: P35813
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... PPM1A(5494)

詳細

詳細

Protein phosphatase 1A, also known as Protein phosphatase 2C isoform alpha (PP2C-alpha) or Protein phosphatase IA, and encoded by the gene name PPM1A, is a member of the PP2C family. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis.

免疫原

Purified recombinant fragment of human PPM1A expressed in E. Coli

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
代謝ホルモン及び受容体

Anti-PPM1A, clone 7F12 Antibody is an antibody against PPM1A for use in western blotting, flow cytometry & ELISA.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected PPM1A in HeLa cells.

ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

品質

Evaluated by Western Blotting in Jurkat, A431, HeLa, Raji, MCF-7 and COS7 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PPM1A in Jurkat, A431, HeLa, Raji, MCF-7 and COS7 cell lysates.

ターゲットの説明

~43 kDa observed. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal in PBS containing up to 0.1% sodium azide and 0.5% protein stabilizer.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable