MABS2283

Anti-PIP2 Antibody, clone KT10

• 別名: Phosphatidylinositol 4;5-bisphosphate;PtdIns(4;5)P2
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: KT10, monoclonal
• 分子量: calculated mol wt 1.042 kDa
• 精製方法: using Protein A
• 化学種の反応性: rat, mouse, human
• 化学種の反応性（ホモロジーによる予測）: bovine
• 包装: antibody small pack of 100 μg
• テクニック: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; inhibition assay: suitable
• アイソタイプ: IgG2a
• エピトープ配列: Unknown
• タンパク質IDアクセッション番号: Unknown
• UniProtアクセッション番号: N/A
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Stag1(20842)

詳細

詳細

PI(4,5)P2 (PIP2) is synthesized mainly by the phosphorylation of PI by PI 4- kinases that produce phosphatidylinositol 4-phosphate (PI4P), which is then phosphorylated by PI4P 5-kinase (PIP5K). Some PIP2 can also be generated through dephosphorylation of PI(3,4,5)P3 by PTEN. Phosphoryl transfer to positions 4 and 5 in PIP2 requires ATP and the cofactor Mg2+. Although most protein kinases achieve maximal active state at low-micromolar intracellular concentrations of ATP, lipid kinases generally require much higher concentrations of ATP to support their activity. Following its synthesis, PIP2 modulates the functions of various proteins including actin-regulating proteins and ion channels. It also serves as a substrate for phospholipase C (PLC) and a source for second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) that are involved in activation and membrane localization of protein kinase C and in the modulation of ion channel activity in endothelial cells and smooth muscle cells. G-protein (Gq) activation that can result in rapid activation of PLC, can reduce PIP2 levels in cells within seconds. The hydrolysis of PIP2 is shown to be enhanced in cells transformed by oncogenes and tumor viruses. Clone KT10 binds to PIP2 even at picogram levels; however, it does not react with PIP or PI even at microgram levels. It is shown to inhibit the proliferation of Src- and erbB-transformed cells but do not affect the proliferation of untransformed cells. It is also reported to abolish PDGF-induced mitogenesis in both untransformed and ras-transformed cells but does not affect the basal level of mitogenesis. (Ref.: Yoneda, A., et al. (2020). Biochem. Biophys. Res. Commun. 527(4); 1050-1056; Harraz, OF., et al. (2020). Proc. Natl. Acad. Sci. USA. 117(34); 20378-20389; Fukami, K., et al. (1988). Proc. Natl. Acad. Sci. USA. 85(23); 9057-9061).

特異性

Clone KT10 is a mouse monoclonal antibody that specifically detects PI(4,5)P2 (PIP2).

免疫原

Liposomes containing dimyristoyl phosphatidylcholine, cholesterol, PIP2 from bovine spinal cord, and lipid A.

アプリケーション

Quality Control Testing

Evaluated by Immunocytochemistry in K562 cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected PI(4,5)P2 (PIP2) in K562 cells.

Tested Applications

ELISA Analysis: A representative lot detected PIP2 in ELISA applications (Fukami, K. et al. (1988). Proc Natl Acad Sci USA. 85(23): 9057-61).

Immunocytochemistry Analysis: A representative lot detected PIP2 in Immunocytochemistry applications (Yoneda, A., et al. (2020). Biochem Biophys Res Commun. 527(4): 1050-1056).

Flow Cytometry Analysis: Analysis: A representative lot detected PIP2 in Flow Cytometry applications (Yoneda, A., et al. (2020). Biochem Biophys Res Commun. 527(4): 1050-1056).

Inhibition Assay: A representative lot of this antibody inhibited intracellular breakdown of PIP2 and also blocked the proliferation of Src- and erbB-transformed cells. (Fukami, K. et al. (1988). Proc Natl Acad Sci USA. 85(23): 9057-61).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-PIP2, clone KT10, Cat. No. MABS2283, is a mouse monoclonal antibody that detects phosphatidylinositol 4,5-bisphosphate (PIP2) and is tested for use in ELISA, Flow Cytometry, Immunocytochemistry, and Inhibition Assay.

物理的形状

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Recommended storage: +2°C to +8°C.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1