MABS2238

Anti-ELOVL5 Antibody, clone Z88

• 別名: Elongation of very long chain fatty acids protein 5;EC:2.3.1.199;3-keto acyl-CoA synthase ELOVL5;ELOVL fatty acid elongase 5;ELOVL FA elongase 5;Fatty acid elongase 1;hELO1;Very long chain 3-ketoacyl-CoA synthase 5;Very long chain 3-oxoacyl-CoA synthase 5
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: Z88, monoclonal
• 分子量: calculated mol wt 35.29 kDa; observed mol wt ~38 kDa
• 精製方法: using protein G
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: monkey, bovine
• 包装: antibody small pack of 100 μg
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• エピトープ配列: C-terminal
• タンパク質IDアクセッション番号: NP_001229757
• UniProtアクセッション番号: Q9NYP7
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ELOVL5(60481)

詳細

詳細

Elongation of very long chain fatty acids protein 5 (UniProt: EC:2.3.1.199, 3-keto acyl-CoA synthase ELOVL5, ELOVL fatty acid elongase 5, ELOVL FA elongase 5, Fatty acid elongase 1, hELO1, Very long chain 3-ketoacyl-CoA synthase 5, Very long chain 3-oxoacyl-CoA synthase 5) is encoded by the ELOVL5 (also known as EVOVL2, PRO0530) gene (Gene ID: 60481) in human. ELOVL5 is a widely expressed, multi-pass membrane protein found in the endoplasmic reticulum (ER). Higher expression of ELOVL5 is reported in the adrenal gland and testis. It catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. It exhibits marked preference for polyunsaturated molecules. It allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Significantly higher levels of ELOVL5 are reported in prostate cancer compared to nonmalignant prostate. ELOVL5 depletion is reported to markedly alter mitochondrial morphology and function that leads to excess generation of reactive oxygen species and results in suppression of prostate cancer cell proliferation, in vivo tumor growth, and metastasis. ELOVL5 is expressed in the brain in a region- and cell type-specific manner and is abundant in the cerebellum, brainstem, and primary and accessory olfactory regions. Mutations of ELOVL5 gene are known to cause the spino-cerebellar ataxia type 38 (SCA38), a rare autosomal neurological disease characterized by gait abnormality, dysarthria, dysphagia, hyposmia, and peripheral neuropathy. (Ref.: Balbo, I., et al. (2021). Front. Neuroanat. 15; 669073; Centenera, MM., et al. (2021). Cancer Res. 81(7); 1704-1718; Alnabulsi, A., et al. (2019). Int. J. Cancer. 145(4);1138-1147).

特異性

Clone Z88 is a mouse monoclonal antibody that detects Elongation of very long chain fatty acids protein 5 (ELOVL5). It targets an epitope within 9 amino acids from the C-terminal region.

免疫原

Ovalbumin-conjugated linear peptide corresponding to 9 amino acids from the C-terminal region of human Elongation of very long chain fatty acids protein 5 (ELOVL5).

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in MCF-7 cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ELOVL5 in MCF-7 cell lysates.

Tested Applications

Western Blotting Analysis: A representative lot detected ELOVL5 in Western Blotting applications (Alnabulsi, A., et al. (2019). Int J Cancer. 145(4):1138-1147).

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ELOVL5 in human prostate tissue sections.

ELISA Analysis: A representative lot detected ELOVL5 in ELISA applications (Alnabulsi, A., et al. (2019). Int J Cancer. 145(4):1138-1147).

Immunohistochemistry Applications: A representative lot detected ELOVL5 in Immunohistochemistry applications (Alnabulsi, A., et al. (2019). Int J Cancer. 145(4):1138-1147).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-ELOVL5 clone Z88, Cat. No. MABS2238, is a mouse monoclonal antibody that detects ELOVL5 and is tested for use in ELISA, Immunohistochemistry, and Western Blotting.

物理的形状

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Recommended storage: +2°C to +8°C.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable