MABS2046

Anti-ApoB Antibody, clone 2G11

clone 2G11, from mouse

• 別名: Apolipoprotein B-100
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 2G11, monoclonal
• 化学種の反応性: hamster, mouse, rat
• 以下との反応禁止: human
• 包装: antibody small pack of 25 μg
• テクニック: ELISA: suitable; western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_033823.2
• UniProtアクセッション番号: E9Q414
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: mouse ... Apob(238055)

詳細

詳細

Apolipoprotein B-100 (UniProt: E9Q414; also known as Apo B-100) is encoded by the Apob gene (Gene ID: 238055) in murine species. Apolipoprotein B is a major protein constituent of chylomicrons. Apo B particles are made up of lipids and proteins and each Apo B is bordered by a phospholipid monolayer with an inner core composed of variable amounts of triglycerides and cholesterol esters. Apo B is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved off. Two isoforms of Apo B are produced- Apo B-100 is synthesized in the liver and Apo B-48 is produced in the intestine. Apo B-100 contains the region that binds to the LDL receptor. It contains VLDL particles, VLDL remnant particles and LDL particles. On the other hand, the Apo B-48 particles are made of chylomicrons and chylomicron remnant particles. Generally, LDL particles account for approximately 90% of Apo B particles in plasma. Abnormal trapping of an Apo B is a major factor in the development of atherosclerotic lesions. When Apo B particles degrade cholesterol is retained with macrophages that induce inflammatory response. Further entry and entrapment of Apo B can promote cardiovascular pathology over time. (Ref.: Sniderman, A et al. (2010). Nat. Rev. Endocrinol. 6(6); 335-346).

特異性

Clone 2G11 detects Apolipoprotein B in Mouse, Rat, and Hamster plasma and tissues. It binds to ApoB-48 and ApoB-100, but not to ApoB-39.

免疫原

ApoB-containing lipoproteins isolated from Apoe -/- Apob 48/48 mice.

アプリケーション

Research Category
細胞シグナル伝達

Detects Apolipoprotein B using this mouse monoclonal Anti-ApoB, clone 2G11 , Cat. No. MABS2046, is a mouse monoclonal antibody that detects Apolipoprotein B in Mouse, Rat, and Hamster and is tested for use in ELISA and Western Blotting.

Western Blotting Analysis: 4 µg/mL from a representative lot detected ApoB in 10 µg of rat liver and mouse liver tissue lysate.

Western Blotting Analysis: A representative lot detected ApoB in Western Blotting applications (Nguyen, A.T., et. al. (2006). Biochem Biophys Acta. 1761(2):182-5; Cheng, D., et. al. (2016). J Biol Chem. 291(45):23793-23803).

ELISA Analysis: A representative lot detected ApoB in ELISA applications (Nguyen, A.T., et. al. (2006). Biochem Biophys Acta. 1761(2):182-5).

品質

Evaluated by Western Blotting in rat serum.

Western Blotting Analysis: 4 µg/mL of this antibody detected ApoB in 10 µg of rat serum.

ターゲットの説明

~260 kDa observed; 509.43 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): does not flash
• 引火点(℃): does not flash