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Key Documents

MABS1904

Sigma-Aldrich

Anti-RAMP1 Antibody, clone 4F8.1

clone 4F8.1, from mouse

別名:

Receptor activity-modifying protein 1, Calcitonin-receptor-like receptor activity-modifying protein 1, CRLR activity-modifying protein 1

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.43

由来生物

mouse

品質水準

抗体製品の状態

purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

4F8.1, monoclonal

化学種の反応性

human

包装

antibody small pack of 25 μg

テクニック

immunohistochemistry: suitable (paraffin)
western blot: suitable

アイソタイプ

IgG2bκ

NCBIアクセッション番号

UniProtアクセッション番号

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... RAMP1(10267)

詳細

Receptor activity-modifying protein 1 (UniProt: O60894; also known as Calcitonin-receptor-like receptor activity-modifying protein 1, CRLR activity-modifying protein 1, RAMP1) is encoded by the RAMP1 gene (Gene ID: 10267) in human. RAMP1 is a single-pass type I membrane protein that is involved in the transports of calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane and acts as a receptor for calcitonin-gene-related peptide (CGRP) together with CALCRL. RAMP1 lacks glycosylation sites and does not migrate to the cell surface independently. To reach the cell surface, it aggregates inside the cytoplasm and forms heterodimers with calcitonin. It is expressed in many tissues including the uterus, bladder, brain, pancreas, retina, and gastro-intestinal tract. It is synthesized with a signal peptide (aa 1-26), which is cleaved to generate the mature form. Mature RAMP1 has an extracellular domain (aa 27-117); a transmembrane domain (aa 118-138); and a short cytoplasmic tail (aa 139-148). RAMP1 is reported to acts as a vasodilation factor and mice deficient in RAMP1 exhibit inflammatory responses with a transient, but significant, increase in serum calcitonin-gene-related peptide levels and pro-inflammatory cytokines. (Ref.: Nakayama, T., et al. (2017). J. Hum. Hypertens. 31(5); 361-365).

特異性

Clone 4F8.1 specifically detects Receptor activity-modifying protein 1 (RAMP1) in human cells. It targets an epitope within 91 amino acids from the internal region.

免疫原

GST/His-tagged recombinant fragment corresponding to 91 amino acids from the internal region of human Receptor activity-modifying protein 1 (RAMP1).

アプリケーション

Research Category
細胞シグナル伝達
Anti-RAMP1, clone 4F8.1, Cat. No. MABS1904, is a mouse monoclonal antibody that detects Receptor activity-modifying protein 1 (RAMP1) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected RAMP1 in human uterus and human colon tissue sections.

品質

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected RAMP1 in human brain tissue lysate.

ターゲットの説明

~17 kDa observed; 16.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

物理的形状

Protein G purified
Format: Purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


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