MABS1320

Anti-Serpin B13 Antibody, clone B29

clone B29, from mouse

• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: B29, monoclonal
• 化学種の反応性: human, mouse
• 包装: antibody small pack of 25 μL
• テクニック: immunofluorescence: suitable; western blot: suitable
• アイソタイプ: IgG2bκ
• NCBIアクセッション番号: NP_766440
• UniProtアクセッション番号: Q8CDC0
• 輸送温度: ambient
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SERPINB13(5275)

詳細

詳細

Serpin B13 (UniProt: Q8CDC0) is encoded by the Serpinb13 gene (Gene ID: 241196) in murine species. Serpin B13 is a cytoplasmic protein that plays a role in the proliferation or differentiation of keratinocytes. Two isoforms of Serpin B13 have been described that are generated by alternative splicing. Serpin B13 acts as a serine proteinase inhibitor and studies have shown a strong induction of serpin B13 in the pancreas of mice during the first few weeks of life. Cathepsin L and K appear to be the targets of serpin B13 action. Serpin B13 is expressed in pancreas with low levels of expression in pancreatic islets and larger expression in the exocrine pancreas. Secretion of anti-serpin B13 autoantibodies in young diabetes-prone non-obese diabetic (NOD) mice is shown to be associated with reduced inflammation in pancreatic islets and a slower progression to autoimmune diabetes. Anti-serpin B13 autoantibodies are shown to protect NOD mice against diabetes before 16 weeks of age. Reduced expression of serpin B13 autoantibodies is associated with development of type I diabetes in human before 5 years of age. Mice injected with monoclonal anti-serpin B13 display few inflammatory cells in the islets and rapid recovery from recent onset diabetes.

特異性

Clone B29 detects Serpin B13 in human and murine species.

免疫原

Purified full length mouse Serpin B13 resuspended in Complete Freund′s Adjuvant.

アプリケーション

Research Category
細胞シグナル伝達

Anti-Serpin B13, clone B29, Cat. No. MABS1320, is a mouse monoclonal antibody that detects Serpin B13 and has been tested for use in Function Studies, Immunofluorescence, and Western Blotting.

Immunofluorescence Analysis: A representative lot detected Serpin B13 in Immunofluorescence applications (Baldzizhar, R., et. al. (2012). J Biol Chem. 288(3):1612-9).

Western Blotting Analysis: 3 ug/mL from a representative lot detected Serpin B13 in mouse serpin/HEK293 cell lysates (Courtesy of Dr. Jan Czyzyk, M.D., University of Rochester Medical Center, NY).

Western Blotting Analysis: A representative lot detected Serpin B13 in Western Blotting applications (Baldzizhar, R., et. al. (2012). J Biol Chem. 288(3):1612-9; Czyzyk, J., et. al. (2012). J Immunol. 188(12):6319-27).

Affects Function Analysis: A representative lot detected Serpin B13 in Affects Function applications (Baldzizhar, R., et. al. (2012). J Biol Chem. 288(3):1612-9).

品質

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .

ターゲットの説明

44.32 kda calculated.

物理的形状

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.