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由来生物
mouse
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
N294A/6, monoclonal
化学種の反応性
rat, human
テクニック
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG2bκ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... BCAN(63827)
詳細
Brevican core protein (BCAN), also known as Brain-enriched hyaluronan-binding protein (BEHAB) or Chondroitin sulfate proteoglycan 7 (CSPG7), may play a role in the terminally differentiating and the adult nervous system during postnatal development. It may stabilize interactions between hyaluronan (HA) and brain proteoglycans. Two isoforms exist, with isoform 1 (~99 kDa) being highly expressed from birth through 8 years of age and down-regulated by 20 years of age to low levels that are maintained in the normal adult cortex; and isoform 2 (~72 kDa) being expressed at uniformly low levels throughout development.
免疫原
Recombinant protein corresponding to rat Brevican.
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
ニューロン発生
ニューロン発生
Anti-Brevican, clone N294A/6 is an antibody targeting the Brevican protein, validated for use in WB & IHC.
Western Blot Analysis: 2 µg/mL from a representative lot detected Brevican in 10 µg of human brain tissue lysate.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Brevican in rat midbrain, rat thalamus, and rat cerebellum tissues.
Western Blot Analysis: A representative lot detected Brevican in rat brain tissue lysate and in WT, but not in Brevican knockout mouse brain tissue lysate.
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Brevican in rat midbrain, rat thalamus, and rat cerebellum tissues.
Western Blot Analysis: A representative lot detected Brevican in rat brain tissue lysate and in WT, but not in Brevican knockout mouse brain tissue lysate.
品質
Evaluated by Western Blot in rat brain tissue lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected Brevican in 10 µg of rat brain tissue lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected Brevican in 10 µg of rat brain tissue lysate.
ターゲットの説明
~140 kDa observed. The calculated molecular weight of this protein is 96 kDa A second isoform is known to exist at 69 kDa This protein may be observed at a higher molecular weight due to glycosylation. A proteolyticaly cleaved fragment of Brevican may be observed at ~50 kDa in some tissue lysates.
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
アナリシスノート
Control
Rat brain tissue lysate
Rat brain tissue lysate
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABN491:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
PloS one, 15(6), e0234632-e0234632 (2020-06-20)
Evidence indicate that the brain-specific protein, brevican, is proteolytically cleaved during neurodegeneration, hence positioning fragments of brevican as potential blood biomarkers of neurodegenerative diseases, such as dementia. We aimed to develop two assays capable of detecting the brevican N-terminal (N-Brev)
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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