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Merck
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主要文書

安全性情報

MABN2678

Sigma-Aldrich

Anti-phospho-α-synuclein (Ser129) Antibody, clone PS129

new

別名:

Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor (NACP)

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About This Item

UNSPSCコード:
12352203

由来生物

mouse

品質水準

抗体製品の状態

purified antibody

抗体製品タイプ

primary antibodies

クローン

PS129, monoclonal

分子量

calculated mol wt 14.46 kDa
observed mol wt ~16 kDa

精製方法

using protein G

化学種の反応性

human, mouse

化学種の反応性(ホモロジーによる予測)

bovine, porcine, rat

包装

antibody small pack of 100

テクニック

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

アイソタイプ

IgG2aκ

エピトープ配列

C-terminal

タンパク質IDアクセッション番号

UniProtアクセッション番号

保管温度

2-8°C

遺伝子情報

human ... SNCA(6622)

特異性

Clone PS129 is a mouse monoclonal antibody that specifically detects -synuclein phosphorylated on serine 129.

免疫原

KLH-conjugated linear peptide corresponding to 9 amino acids surrounding phosphoserine 129 from the C-terminal region of human -synuclein.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting with recombinant -synuclein phosphorylated S129 and recombinant -synuclein monomers.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected -synuclein phosphorylated on serine 129 in recombinant -synuclein phosphorylated S129 protein, but not in recombinant -synuclein monomers.

Tested Applications

ELISA Analysis: A representative lot detected -synuclein in ELISA application (Majbour, N.K., et al. (2016). Mol Neurodegener. 11:7; El-Agnaf, O., et al. (2017). Neurobiol Dis. 104: 85-96).

Immunohistochemistry Application: A representative lot detected phospho- -synuclein (Ser129) in Immunohistochemistry application (Vaikath, N.N., et al. (2015). Neurobiol Dis. 79:81-99).

Western Blotting Analysis: A representative lot detected phospho- -synuclein (Ser129) in Western Blotting application (Majbour, N.K., et al. (2016). Mol Neurodegener. 11:7).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

Alpha-synuclein (UniProt: P37840; also known as Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP) is encoded by the SNCA (also known as NACP, PARK1) gene (Gene ID: 6622) in human. Alpha-synuclein is a membrane-bound protein in dopaminergic neurons that is involved in the regulation of dopamine release and transport. It is also reported to induce fibrillization of microtubule-associated protein tau and reduces neuronal responsiveness to various apoptotic stimuli. It is primarily expressed in the brain and is associated with the regulation of neurotransmitter release at synapses. Alpha-synuclein is also found in the cytoplasm, where it exists in a dynamic equilibrium between a soluble cytosolic form and a membrane-bound form. It associates with lipid membranes, particularly those of synaptic vesicles. Aggregation of alpha-synuclein is known to be a causative factor in the development of Parkinson′s disease (PD), which leads to the degeneration of dopaminergic neurons in the substantia nigra (SN) in the midbrain region. -synuclein can be phosphorylated, predominantly on serine residues by casein kinase 1 (CK1). Phosphorylation by CK1 appears to occur on residue (serine 129) distinct from the residue (tyrosine 125) phosphorylated by other kinase FYN. The phosphorylation of -synuclein at serine 129 (pS129) has been extensively studied due to its significant implications in neurodegenerative diseases, particularly Parkinson′s disease (PD) and other synucleinopathies. Phosphorylation of Ser129 is reported to be selective and extensive in synucleinopathy lesions and promotes insoluble fibril formation. This serves as a potential biomarker for disease diagnosis and progression and offers a promising target for therapeutic intervention. (Ref.: Vaikath, N.N., et al. (2015). Neurobiol Dis. 79:81-99; Majbour, N.K., et al. (2016). Mol Neurodegener. 19:11:7; El-Agnaf, O., et al. (2017). Neurobiol Dis. 104:85-96).

物理的形状

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

再構成

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Recommended storage: +2°C to +8°C.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

MABN2678-100UG:
MABN2678-25UG:


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