MABN2616

Anti-TMEM30A Antibody, clone 7F4

• 別名: Cell cycle control protein 50A;CDC50A;P4-ATPase flippase complex beta subunit;Transmembrane protein 30A
• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 7F4, monoclonal
• 分子量: calculated mol wt 40.68 kDa; observed mol wt ~52-54 kDa
• 精製方法: using protein G
• 化学種の反応性: human, mouse
• 化学種の反応性（ホモロジーによる予測）: feline, porcine, canine, monkey, horse, bovine
• 包装: antibody small pack of 100 μg
• テクニック: immunohistochemistry (frozen sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• エピトープ配列: N-terminal
• タンパク質IDアクセッション番号: NP_001068691
• UniProtアクセッション番号: Q17QL5
• 輸送温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: bovine ... TMEM30A(505797)

詳細

詳細

Cell cycle control protein 50A (UniProt: Q17QL5; also known as P4-ATPase flippase complex beta subunit TMEM30A, Transmembrane protein 30A) is encoded by the TMEM30A gene (Gene ID: 505797) in bovine species. TMEM30A is a multi-pass membrane protein with two cytoplasmic domains, two transmembrane domains, and one extracellular domain. It is expressed in photoreceptor cells and is detected in retina outer segment and other retinal layers. It is a component of various P4-ATPase flippase complexes, which consists of a catalytic -subunit and an accessory β-subunit. It catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. The β-subunit may assist in binding of the phospholipid substrate. Most P4-ATPases associate with one of three accessory subunit isoforms known as CDC50A (TMEM30A), CDC50B (TMEM30B), and CDC50C (TMEM30C). TMEM30A is required for the proper folding, assembly and endoplasmic reticulum (ER) to Golgi exit of the ATP8A2:TMEM30A flippase complex. This ATP8A2:TMEM30A complex may be involved in regulation of neurite outgrowth. It is also involved in the uptake of platelet-activating factor (PAF). (Ref.: Liou, AY., et al. (2019). J. Biol. Chem. 294(17); 6809-6821; Wang, J., et al. (2018). Sci. Rep. 8: 10795).

特異性

Clone 7F4 is a mouse monoclonal antibody that detects Cell cycle control protein 50A (TMEM30A). It targets an epitope within 9 amino acids from the N-terminal, cytoplasmic domain.

免疫原

A linear peptide corresponging to 9 amino acids from the N-terminal region of bovine cell cycle control protein 50A (TMEM30A).

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in Mouse testis tissue lysate.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected TMEM30A in Mouse testis tissue lysate.

Tested applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected TMEM30A in Human brain, mouse brain, and mouse liver tissue lysates.

Immunoaffinity Purification: A representative lot was used in Immunoaffinity Purification of TMEM30A. (Wang, J., et al. (2018). Sci Rep. 8(1):10795).

Immunohistochemistry Applications: A representative lot detected TMEM30A in Immunohistochemistry applications (Coleman, J.A., et al. (2011). J Biol Chem. 286(19):17205-16).

Western Blotting Analysis: A representative lot detected TMEM30A in Western Blotting applications (Coleman, J.A., et al. (2011). J Biol Chem. 286(19):17205-16; Wang, J., et al. (2018). Sci Rep. 8(1):10795; Liou, A.Y., et al. (2019). J Biol Chem. 294(17):6809-6821).

Immunoprecipitation Analysis: A representative lot detected TMEM30A in Immunoprecipitation applications (Coleman, J.A., et al. (2011). J Biol Chem. 286(19):17205-16).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-TMEM30A, clone 7F4, Cat. No. MABN2616, is a mouse monoclonal antibody that detects TMEM30A and is tested for use in Immunohistochemistry, Immunoaffinity Purification, Immunoprecipitation, and Western Blotting.

物理的形状

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Recommended storage: +2°C to +8°C.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable