MABN122

Anti-Robo2 Antibody, clone 4C6.1

clone 4C6.1, from mouse

• 別名: Roundabout homolog 2
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 4C6.1, monoclonal
• 化学種の反応性: mouse, human, rat
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_002933
• UniProtアクセッション番号: Q9HCK4
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ROBO2(6092)

詳細

詳細

Roundabout homolog 2 (Robo2) is a receptor for SLIT2, and likely SLIT1, which are thought to help guide cellular migration, including axonal navigation of the neural tube and the projection of axons during neuronal development. Vesicoureteral reflux type 2 (VUR2) has been attributed to defects in Robo2.

特異性

This antibody recognizes the cytoplasmic domain of Robo2

免疫原

Epitope: Cytoplasmic domain

GST-tagged recombinant protein corresponding to the cytoplasmic domain of human Robo2.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
発生シグナル伝達

Detect Roundabout homolog 2 using this mouse monoclonal antibody, Anti-Robo2 Antibody, clone 4C6.1 validated for use in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Robo2 in rat brain (hypothalamus) and human brain tissue.

品質

Evaluated by Western Blotting in mouse neuronal stem cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Robo2 in 200 µg of mouse neuronal stem cell lysate.

ターゲットの説明

~ 170 kDa observed. The calculated molecular weight is 150 kDa, however Robo2 has been shown as a ~170 kDa band in western blots due to high glycosylation. (Fricke, C., et al. (2001). Science. 292:(5516):507-510).

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Mouse neuronal stem cell lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable