MABN109

Anti-Synaptotagmin-4 Antibody, clone 10C11.1

clone 10C11.1, from mouse

• 別名: Synaptotagmin-4, Synaptotagmin IV, SytIV
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 10C11.1, monoclonal
• 化学種の反応性: human, mouse
• テクニック: immunohistochemistry: suitable (paraffin); western blot: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_065834
• UniProtアクセッション番号: Q9H2B2
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SYT4(6860)

詳細

詳細

Synaptotagmin-4 (UniProt Q9H2B2; also known as Synaptotagmin IV, SytIV) is encoded by the SYT4 (also known as KIAA1342) gene (Gene ID 6860) in human. The mammalian synaptotagmin family is comprised of 17 members. While most of them are predominantly function in the cetnral nervous system (CNS), some are involved in the vesicular functions of endocrine cells and glucose-transport metabolic cells. Synaptotagmin-4 is an inducible member detectable only in the brain and the neuroendocrine system. Unlike other family members, synaptotagmin-4 does not bind calcium and does not medidate calcium-induced exocytosis. Instead, synaptotagmin-4 is shown to play a neuroendocrine role in inhibiting exocytotic activities in the posterior pituitary and in cultured PC12 cells. Synaptotagmin-4 is expressed most abundantly in oxytocin neurons of the hypothalamus, where it negatively regulates oxytocin exocytosis. Dietary obesity is associated with increased vesicle binding of synaptotagmin-4 and decreased oxytocin release. Normalizing oxytocin release via Synaptotagmin-4 inhibition prevents against dietary obesity under chronic nutritional excess. Human synaptotagmin-4 consists of a vesiclular domain (a.a. 1-16), a transmembrane segment (a.a. 17-37),and a large cytoplasmic domain (a.a. 38-425).

特異性

Clone 10C11.1 targets a cytoplasmic epitope present in both human spliced isoforms reported by UniProt (Q9H2B2).

免疫原

Epitope: Cytoplasmic domain.

GST-tagged recombinant human Synaptotagmin-4 cytoplasmic domain fragment.

アプリケーション

Research Category
ニューロサイエンス

Research Sub Category
小胞輸送

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Synaptotagmin-4 in human kidney, skeletal muscle, and pancreas tissue sections.

This Anti-Synaptotagmin-4 Antibody, clone 10C11.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Synaptotagmin-4.

品質

Evaluated by Western Blotting in mouse cerebellum tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Synaptotagmin-4 in 10 µg of mouse cerebellum tissue lysate.

ターゲットの説明

~48 kDa observed. 47.96/46.06 kDa (human isoform 1/2) and 47.66 kDa (mouse) calculated.

物理的形状

Protein G purified.

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1