詳細
Endoplasmic reticulum aminopeptidase 1 (UniProt Q9NZ08; also known as A-LAP, Adipocyte-derived leucine aminopeptidase, Aminopeptidase PILS, ARTS-1, Endoplasmic reticulum aminopeptidase associated with antigen processing, PILS-AP, Puromycin-insensitive leucyl-specific aminopeptidase, Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator) is encoded by the ERAP1 (also known as ALAP, APPILS, ARTS1, ERAAP, ERAAP1, KIAA0525, PILSAP) gene (ORF UNQ584/PRO1154; Gene ID 51752) in human. Endoplasmic reticulum aminopeptidase 1 (ERAP1) is a single transmembrane protein belonging to the M1 family of zinc metallopeptidases. ERAP1 consists of an N-terminal transmembrane segment (a.a. 2-21) and a large lumenal domain (a.a. 22-941), with only the N-terminus methionine exposed cytoplasmically. ERAP1 processes peptides for major histocompatibility complex (MHC) class I presentation and promotes ectodomain shedding of several cytokine receptors, including TNF-R1, IL-6R alpha chain, and IL-1 type II decoy receptor (IL-1RII). Ankylosing spondylitis (AS) is a common, highly heritable form of inflammatory arthritis. Several ERAP1 single nucleotide polymorphic (SNP) variants are reported to influence the risk of AS in individuals carrying class I MHC allele HLA-B*27 and HLA-B*40:01.
特異性
Expected to react with both spliced isoforms of human ERAP1 (UniProt Q9NZ08-1 & Q9NZ08-2)
免疫原
Epitope: Internal.
GST-tagged recombinant human ERAP1 internal fragment.
アプリケーション
Research Category
炎症及び免疫
Research Sub Category
炎症及び自己免疫疾患
Detect ERAP1 using this Anti-ERAP1 Antibody, clone 16A7.1 validated for use in Western Blotting.
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected ERAP1 in 10 µg of K562 cell lysate.
品質
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected ERAP1 in 10 µg of A431 cell lysate.
ターゲットの説明
~110 kDa observed. 107.2 kDa (isoform 1) and 107.8 kDa (Isoform 2) calculated.
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
