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由来生物
rat
結合体
FITC conjugate
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
30-F11, monoclonal
交差性
mouse
テクニック
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
アイソタイプ
IgG2bκ
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... PTPRC(5788)
関連するカテゴリー
詳細
CD45, also known as PTPRC, is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells. CD 45 belongs to the protein-tyrosine phosphatase family, receptor class 1/6 subfamily.It also contains 2 fibronectin type-III domains and 2 tyrosine-protein phosphatase domains. CD45 has been shown to play an important role in T-cell activation through the antigen receptor. CD45 also is suggested to act as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one is suggested to affect the substrate specificity of the first domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN. Furthermore, it has been shown to dephosphorylate LYN, and modulate LYN activity. Interestingly, CD45 has been associated with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45. CD45 has been shown to bind with GANAB and PRKCSH. Additionally, it interacts with SKAP1 and DPP4, whereas the interaction is enhanced in a interleukin-12-dependent manner in activated lymphocytes. Mutations in CD45 have been associated with Multiple Sclerosis (MS) and severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.
免疫原
Mouse thymus or spleen
アプリケーション
Research Category
炎症及び免疫
炎症及び免疫
Research Sub Category
サイトカイン及びサイトカイン受容体
サイトカイン及びサイトカイン受容体
Anti-CD45 (mouse) Antibody, FITC, clone 30-F11 is a highly specific rat monoclonal antibody, that targets CD45 & has been tested in Flow Cytometry, IHC, western blotting & IP.
Western Blotting Analysis: A representative lot detected human CD45 in Western Blotting. (Tsuboi S and Fukuda M. 1998)
Immunohistochemistry Analysis: A representative lot detected human CD45 in Immunohistochemistry. (Simon DI, et al. 2000; Cornet A, et al. 2001)
Immunoprecipitation Analysis: A representative lot detected human CD45 in Immunoprecipitation. (Ledbetter JA, et al. 1979)
Immunohistochemistry Analysis: A representative lot detected human CD45 in Immunohistochemistry. (Simon DI, et al. 2000; Cornet A, et al. 2001)
Immunoprecipitation Analysis: A representative lot detected human CD45 in Immunoprecipitation. (Ledbetter JA, et al. 1979)
品質
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC and unconjugated antibody.
Flow Cytometry Analysis: 0.25 μg of this antibody detected CD45 in one million C57BL/6 mouse splenocytes.
Flow Cytometry Analysis: 0.25 μg of this antibody detected CD45 in one million C57BL/6 mouse splenocytes.
ターゲットの説明
145 kDa calculated
物理的形状
Affinity
Purified rat conjugate antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide.
保管および安定性
The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABF320:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Binhui Zhou et al.
Frontiers in immunology, 11, 607442-607442 (2021-01-26)
Zdhhc family genes are composed of 24 members that regulate palmitoylation, a post-translational modification process for proteins. Mutations in genes that alter palmitoylation or de-palmitoylation could result in neurodegenerative diseases and inflammatory disorders. In this study, we found that Zdhhc2
Overexpression of branched O-linked oligosaccharides on T cell surface glycoproteins impairs hu?moral immune responses in transgenic mice.
Tsuboi, S and Fukuda, M
The Journal of Biological Chemistry, 273, 30680-30687 (1998)
D I Simon et al.
The Journal of clinical investigation, 105(3), 293-300 (2000-02-17)
Inflammation plays an essential role in the initiation and progression of atherosclerosis, but its role in vascular repair after mechanical arterial injury (i.e., percutaneous transluminal coronary angioplasty, PTCA) is unknown. In animal models of vascular injury, leukocytes are recruited as
A Cornet et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(23), 13306-13311 (2001-11-01)
Early pathological manifestations of Crohn's disease (CD) include vascular disruption, T cell infiltration of nerve plexi, neuronal degeneration, and induction of T helper 1 cytokine responses. This study demonstrates that disruption of the enteric glial cell network in CD patients
Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens.
J A Ledbetter et al.
Immunological reviews, 47, 63-90 (1979-01-01)
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