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由来生物
rat
品質水準
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
5A8, monoclonal
化学種の反応性
mouse
包装
antibody small pack of 100
テクニック
activity assay: suitable
direct ELISA: suitable
western blot: suitable
アイソタイプ
IgG1κ
エピトープ配列
Unknown
タンパク質IDアクセッション番号
UniProtアクセッション番号
遺伝子情報
mouse ... Klrk1(24099)
特異性
Clone 5A8 is a rat monoclonal antibody that detects B-cell activating factor (BAFF/CD257).
免疫原
Rat RBL cells expressing full-length mouse BAFF.
アプリケーション
Quality Control Testing
Evaluated by Western Blotting with recombinant murine BAFF protein .
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected recombinant murine BAFF/CD257 protein .
Tested Applications
Activity Assay: A representative lot activated mouse BAFF in a reporter cell assay, in primary B cells. (Kowalczyk-Quintas, C., et al. (2016). J Biol.Chem.291(38):19826-34; Vigolo, M., et al. (2018). Nat Commun.9(1):1199).
ELISA Analysis: A representative lot detected BAFF/CD257 in ELISA applications (Batten, M., et al. (2004). J Immunol. 172(2):812-22; Vigolo, M., et al. (2018). Nat Commun. 9(1):1199; Kowalczyk-Quintas, C., et al. (2016). J Biol Chem. 291(38):19826-34).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with recombinant murine BAFF protein .
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected recombinant murine BAFF/CD257 protein .
Tested Applications
Activity Assay: A representative lot activated mouse BAFF in a reporter cell assay, in primary B cells. (Kowalczyk-Quintas, C., et al. (2016). J Biol.Chem.291(38):19826-34; Vigolo, M., et al. (2018). Nat Commun.9(1):1199).
ELISA Analysis: A representative lot detected BAFF/CD257 in ELISA applications (Batten, M., et al. (2004). J Immunol. 172(2):812-22; Vigolo, M., et al. (2018). Nat Commun. 9(1):1199; Kowalczyk-Quintas, C., et al. (2016). J Biol Chem. 291(38):19826-34).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
ターゲットの説明
Tumor necrosis factor ligand superfamily member 13B (UniProt: Q9WU72; also known as B-cell-activating factor, BAFF, CD257) is encoded by the Tnfsf13b (also known as Baff) gene (Gene ID: 24099) in murine species. BAFF is a single-pass type II, homotrimeric, membrane glycoprotein with a cytoplasmic domain (aa 1-47), a transmembrane domain (aa 48-68), and an extracellular domain (aa 69-309). A soluble form of BAFF has also been described that is produced by the cleavage of membrane form between amino acids 126-127. It is expressed by cells of myeloid origin and by stromal cells. Two isoforms of BAFF have been described that are produced by alternative splicing. Isoform 2 is reported to inhibit the secretion and bioactivity of isoform 1. BAFF is reported to bind to TNFRSF13B/TACI and TNFRSF17/BCMA. It serves as a potent B cell survival factor and is a critical immune checkpoint during peripheral B cell maturation. Its levels are often elevated in autoimmune diseases, such as rheumatoid arthritis and systemic lupus erythematosus (SLE). Elevated levels of BAFF are also observed in subjects with non-Hodgkin s lymphoma (NHL) and it is reported to promote survival of both NHL-derived B lymphoma cells and B cell chronic lymphocytic leukemia (B-CLL) cells. (Ref.: Vigolo, M., et al. (2018). Nat. Commun. 9(1); 1199; Kowalczyk-Quintas, C., et al (2016). J. Biol. Chem. 291(38); 19826-19834; Batten, M., et al. (2004). J. Immunol. 172(2); 812-822; Gavin, AL., et al. (2003). J. Biol. Chem. 278(40); 38220-38228).
物理的形状
Purified rat monoclonal antibody IgG1 in PBS without azide.
再構成
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
保管および安定性
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABF3094-100UL:
MABF3094-25UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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