MABF3048

Anti-MERS-CoV Nucleoprotein Antibody

• UNSPSCコード: 12352203
• NACRES: NA.43

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 46, monoclonal
• 分子量: calculated mol wt 45.05 kDa; observed mol wt ~45 kDa
• 交差性: virus
• 包装: antibody small pack of 100 μL
• テクニック: ELISA: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG1κ
• UniProtアクセッション番号: R9UM87
• 輸送温度: dry ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: Unknown ... N nucleocapsid protein(14254601)

詳細

詳細

Nucleoprotein (UniProt: R9UM87; also known as Nucleocapsid protein, NC, Protein N) is encoded by the N gene (Gene ID: 14254601) in Middle East respiratory syndrome-related coronavirus (MERS-CoV). The MERS-CoV is a positive-strand RNA virus of the betacoronavirus genus within the Coronaviridae family that causes severe respiratory syndrome in human. The mature MERS-CoV contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). Nucleoprotein of MERS-CoV is a homooligomeric multifunctional protein that plays roles in genome encapsidation, RNA synthesis during replication, host translational shutoff, and innate immunity disruption. It packages the positive strand viral genome RNA into helical ribonucleocapsid and is involved in virion assembly through its interactions with the viral genome and membrane protein M. The nucleoprotein is reportedly organized into two folded domains known as the N-terminal domain (NTD) and the C-terminal domain (CTD) that are separated by a disordered region (LKR) containing a serine/ arginine stretch. Clone 46 displays specificity for MERS-CoV nucleoprotein and does not show cross-reactivity with nucleoprotein from other related viruses, including SARS coronavirus. (Ref.: Nguyen, THV., et al. (2019). Acta Crystallogr. D. Struct. Biol. 75(1); 8-15; Yamaoka, Y., et al. (2016). Front. Microbiol. 7; Article 509).

特異性

Clone 46 is a mouse monoclonal antibody that detects nucleoprotein from Middle East respiratory syndrome-related coronavirus (MERS-CoV).

免疫原

His-tagged recombinant fragment corresponding to 292 amino acids from the nucleoprotein of Middle East respiratory syndrome-related coronavirus (MERS-CoV).

アプリケーション

Quality Control Testing

Evaluated by Western Blotting with recombinant MERS-CoV nucleoprotein.

Western Blotting Analysis: A 1:50,000 dilution of this antibody detected recombinant MERS-CoV Nucleoprotein.

Tested Applications

ELISA Analysis: A representative lot detected MERS-CoV Nucleoprotein in ELISA applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

Western Blotting Analysis: A representative lot detected MERS-CoV Nucleoprotein in Western Blotting applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

Immunoprecipitation Analysis: A representative lot immunoprecipitated MERS-CoV Nucleoprotein in Immunoprecipitation applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-MERS-CoV Nucleoprotein, clone 46, Cat. No. MABF3048, is a mouse monoclonal antibody that detects MERS-CoV Nucleoprotein and is tested for use in ELISA, Immunoprecipitation, and Western Blotting.

物理的形状

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at +2°C to +8°C from date of receipt.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable