由来生物
mouse
品質水準
結合体
unconjugated
抗体製品の状態
purified antibody
抗体製品タイプ
primary antibodies
クローン
MH9A4, monoclonal
分子量
observed mol wt ~57 kDa
精製方法
using protein G
化学種の反応性
human
包装
antibody small pack of 100 μL
テクニック
ELISA: suitable
flow cytometry: suitable
アイソタイプ
IgG2b
エピトープ配列
Unknown
タンパク質IDアクセッション番号
UniProtアクセッション番号
輸送温度
2-8°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
mouse ... IL9(3578)
詳細
Interleukin-9 (UniProt: P15248; also known as IL-9, Cytokine P40, T-cell growth factor P40) is encoded by the IL9 gene (Gene ID: 3578) in human. IL-9 is a pleiotropic cytokine that plays a significant role in tolerance and inflammation. It is expressed by a variety of immune cells. It is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form. It supports the growth of mast cells, T cells, and several other cell types, including erythroid progenitors, fetal thymocytes, human megakaryoblastic leukemic cells, and myeloid precursor cells. It is also reported to supports IL-2 independent and IL-4 independent growth of helper T-cells. It also plays a role in promoting IgE production by B cells. IL-9 actions are mediated via its binding to IL-9R that results in the formation the IL-9R heterocomplex. Following its binding to the IL-9R , the SHC is phosphorylated, which then binds to Grb2 that subsequently activates SoS, a GTP exchange factor for Ras GTPases. This results in the activation of Ras GTPase that activates Raf kinase followed by the activation of MEK1/2 and ERK1/2. Although IL-9 is reported to activate the MAPK pathway in several lymphoid and hematopoietic cells, this activation is weaker compared to other cytokines. Elevated levels of IL-9 and IL-9R are observed in lungs of asthmatic subjects. (Ref.: Chakraborty, S., et al. (2019). Int. J. Mol. Sci. 20(9); 2113).
特異性
Clone MH9A4 is a mouse monoclonal antibody that detects human Interleukin-9.
免疫原
Ovalbumin-conjugated recombinant human Interleukin-9.
アプリケーション
Quality Control Testing
Evaluated by ELISA with recombinant human IL-9.
ELISA Analysis:: Various dilution of this antibody detected recombinant human Interleukin-9 (IL-9).
Tested Applications
Flow Cytometry Analysis: A representative lot detected IL-9 in Flow Cytometry applications (Lozano, E., et al. (2012). J Immunol.;188(8):3869-75; Muls, N., et al. (2012). J Neuroimmunol.;243(1-2):73-80).
ELISA Analysis: A representative lot detected IL-9 in ELISA applications (Jenmalm, M.C., et al. (2001). Clin Exp Allergy.;31(10):1528-35; Faulkner, H., et al. (2002). J Infect Dis.;185(5):665-72; Bottcher, M.F., et al. (2003). Pediatr Allergy Immunol.;14(5):345-50; Holz, L.E., et al. (2005). J Inflamm (Lond).;2:3; Chang, H.C., et al. (2010). Nat Immunol.;11(6):527-34; Stephens, G.L., et al. (2011). Eur J Immunol.;41(4):952-62; Lozano, E., et al. (2012). J Immunol.;188(8):3869-75; Muls, N., et al. (2012). J Neuroimmunol.;243(1-2):73-80).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by ELISA with recombinant human IL-9.
ELISA Analysis:: Various dilution of this antibody detected recombinant human Interleukin-9 (IL-9).
Tested Applications
Flow Cytometry Analysis: A representative lot detected IL-9 in Flow Cytometry applications (Lozano, E., et al. (2012). J Immunol.;188(8):3869-75; Muls, N., et al. (2012). J Neuroimmunol.;243(1-2):73-80).
ELISA Analysis: A representative lot detected IL-9 in ELISA applications (Jenmalm, M.C., et al. (2001). Clin Exp Allergy.;31(10):1528-35; Faulkner, H., et al. (2002). J Infect Dis.;185(5):665-72; Bottcher, M.F., et al. (2003). Pediatr Allergy Immunol.;14(5):345-50; Holz, L.E., et al. (2005). J Inflamm (Lond).;2:3; Chang, H.C., et al. (2010). Nat Immunol.;11(6):527-34; Stephens, G.L., et al. (2011). Eur J Immunol.;41(4):952-62; Lozano, E., et al. (2012). J Immunol.;188(8):3869-75; Muls, N., et al. (2012). J Neuroimmunol.;243(1-2):73-80).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-IL-9, clone MH9A4, Cat. No. MABF2795, is a mouse monoclonal antibody that detects Interleukin-9 and is tested for use in ELISA and Flow Cytometry.
物理的形状
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
保管および安定性
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABF2795-100UL:
MABF2795-25UL:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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