MABF2760

Anti-CD200/OX2 Antibody, clone OX-90

• 別名: MRC OX-2 antigen
• UNSPSCコード: 12352203

特性

• 由来生物: rat
• 品質水準: 200
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: OX-90, monoclonal
• 分子量: calculated mol wt 31.26 kDa
• 精製方法: using protein G
• 化学種の反応性: mouse
• 包装: antibody small pack of 100
• テクニック: ELISA: suitable; blocking: suitable; flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable
• アイソタイプ: IgG2aκ
• エピトープ配列: Extracellular domain
• UniProtアクセッション番号: O54901
• 保管温度: -10 to -25°C
• 遺伝子情報: mouse ... Cd200(17470)

詳細

特異性

Clone OX-90 is a rat monoclonal antibody that detects CD200/OX2. It targets an epitope within the extracellular domain.

免疫原

Mouse CD200 rat-CD4 fusion protein

アプリケーション

Quality Control Testing

Evaluated by Flow Cytometry in BALB/c mouse splenocytes.

Flow Cytometry Analysis: 1 µg from a representative lot detected CD200/OX2 in one million BALB/c mouse splenocytes.

Tested Applications

Flow Cytometry Analysis: A representative lot detected CD200/OX2 in flow cytometry application (Hoek, R.M., et al. (2000). Science. 290(5497):1768-71; Broderick, C., et al. (2002). Am J Pathol. 161(5):1669-77; Akkaya, M., et al. (2013). PLoS One. 8(5):e63325).

Enzyme Immunoassay (ELISA): A representative lot detected CD200/OX2 in ELISA application (Hoek, R.M., et al. (2000). Science. 290(5497):1768-71).

Immunofluorescence Analysis: A representative lot detected CD200/OX2 in immunofluorescence application (Broderick, C., et al. (2002). Am J Pathol. 161(5):1669-77; Snelgrove, R.J., et al. (2008). Nat Immunol. 9(9):1074-83).

Blocking of Interfering Antibodies: A representative lot effectively blocked endogenous binding of CD200/OX2 to CD200R (Snelgrove, R.J., et al. (2008). Nat Immunol. 9(9):1074-83; Akkaya, M., et al. (2013). PLoS One. 8(5):e63325).

Immunohistochemistry Analysis: A representative lot detected CD200/OX2 in immunohistochemistry application (Hoek, R.M., et al. (2000). Science. 290(5497):1768-71; Broderick, C., et al. (2002). Am J Pathol. 161(5):1669-77; Snelgrove, R.J., et al. (2008). Nat Immunol. 9(9):1074-83).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

ターゲットの説明

OX-2 membrane glycoprotein (UniProt: O54901; also known as CD200, MRC OX-2 antigen) is encoded by the Cd200 (also known as Mox2) gene (Gene ID: 17470) in mouse. CD200/OX2 is a widely expressed, single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 31-232), a transmembrane domain (aa 233-259), and a cytoplasmic domain (aa 260-278). It also contains one V-type Ig-like domain (aa 31-141) and one C2-type Ig-like domain (aa 142-232). It is widely expressed on a broad range of cells including lymphoid cells, neurons, and endothelium. It interacts with CD200R, an immunoglobulin superfamily inhibitory receptor that is expressed primarily on myeloid/monocyte lineage cells and has a suppressive effect on T cell-mediated immune response. CD200 also serves as a pani-slet marker of pancreatic endocrine cells, including insulin-expressing β- cells, but does not recognize exocrine cells. Its levels are reported to be elevated in pancreatic β cells following SARS-CoV-2 infection and this infection is strictly dependent on ACE2 receptor. Three isoforms of CD200/OX2 have been described that are produced by alternative splicing. (Ref.: van der Heide, V., et al. (2022). Cell Rep. 38(11); 110508; Dorfman, DM., and Shahsafaei, A. (2010). Am. J. Clin. Pathol. 134(5); 726-733; Dorrell, C., et al. (2008). Stem Cell Res. 1(3); 183-194).

物理的形状

Purified rat monoclonal antibody IgG2a in PBS without azide.

再構成

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

保管および安定性

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable