MABF2096

Anti-Norovirus (MNV-1) Antibody, clone 4F9.4

clone 4F9.4, from mouse

• 別名: MNV-1, Murine Norovirus
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: 4F9.4, monoclonal
• 化学種の反応性: norovirus (murine), virus
• 包装: antibody small pack of 25 μg
• テクニック: ELISA: suitable; neutralization: suitable; western blot: suitable
• アイソタイプ: IgAκ
• UniProtアクセッション番号: Q2V8W4_9CALI
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Norovirus is a member of the Caliciviridae family and is responsible for gastroenteritis outbreaks. Murine norovirus (MNV-1) that is about 28 to 35 nm in diameter, shares many biochemical and genetic features with human noroviruses. An analysis of the MNV-1 genome shows three open reading frames (ORF) that are characteristic of noroviruses and vesiviruses. ORF1 is reported to encode a predicted 187 kDa polyprotein that contains the 2C helicase, 3C protease, and 3D polymerase motifs. ORF2 encodes a 59 kDa capsid protein that can self-assemble into virus-like particles when expressed in a baculovirus expression system. ORF3 is reported to encode a putative 22 kDa basic protein. The norovirus capsid protein contains a highly conserved shell (S) and protruding (P) domain and the P domain is shown to contain binding sites for receptors and neutralizing antibodies. The S domain is shown to be highly conserved and the capsid sequence diversity is limited to the P domain. MNV-1 infection is reported to modulate the MAPK pathway to activate eIF4E phosphorylation and the activation of p38 and Mnk during MNV-1 infection is considered to be important for its replication. This monoclonal antibody, clone 4F9.4, recognizes the P domain portion of the viral capsid protein and effectively neutralize MNV-1 and WU20 strains. It can also neutralize recombinant viruses V339I and D348E at higher doses. (Ref.: Kolawole, AO et al. (2014). J. Gen. Virol. 95(9); 1958-1968).

特異性

Clone 4F9.4 specifically targets capsid protein of murine norovirus.

免疫原

Infectious murine norovirus-1

アプリケーション

Research Category
炎症及び免疫

Anti-Norovirus (MNV-1), clone 4F9.4 Antibody, Cat. No. MABF2096, is a mouse monoclonal antibody that detects Norovirus (MNV-1) and has been tested for use in ELISA, Neutralizing, and Western Blotting.

Neutralizing Analysis: A representative lot neutralized murine norovirus (MNV-1) in Dulbecco s modified Eagle′s medium (DMEM) (Courtesy of Abimbola Kolawole, Ph.D. and Christiane Wobus, Ph.D., University of Michigan Medical School, Ann Arbor, Michigan USA).

ELISA Analysis: A representative lot detected Norovirus (MNV-1) in ELISA applications (Kolawole, A.O., et. al. (2014). J Gen Virol. 95(Pt 9):1958-68).

Neutralizing Analysis: A representative lot effectively neutralized Norovirus (MNV-1) in Neutralizing applications (Kolawole, A.O., et. al. (2014). J Gen Virol. 95(Pt 9):1958-68).

Western Blotting Analysis: A representative lot detected Norovirus (MNV-1) in Western Blotting applications (Kolawole, A.O., et. al. (2014). J Gen Virol. 95(Pt 9):1958-68).

品質

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed to be mouse IgA by isotyping testing.

ターゲットの説明

187.47 kDa calculated.

物理的形状

Purified by size-exclusion chromatography

Format: Purified

Purified mouse monoclonal antibody IgA in PBS with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.