MABF2085

Anti-CD11b/CD18 Antibody, clone mAb107

clone mAb107, from mouse

• 別名: Integrin alpha-M/ITGB2, CR3
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

特性

• 由来生物: mouse
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: mAb107, monoclonal
• 化学種の反応性: nonhuman primates, human
• 包装: antibody small pack of 25 μg
• テクニック: dot blot: suitable; flow cytometry: suitable; immunohistochemistry: suitable; inhibition assay: suitable
• アイソタイプ: IgG1κ
• NCBIアクセッション番号: NP_000623
• UniProtアクセッション番号: P05107; P11215
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ITGAM(3684)

詳細

詳細

Integrin alpha-M (UniProt: P11215; also known as CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b) is encoded by the ITGAM (also known as CD11B, CR3A) gene (Gene ID: 3684) in human. Integrins are alpha/beta heterodimeric adhesion receptors that couple the extracellular matrix on other cells with the contractile cytoskeleton to transduce mechanochemical signals across the plasma membrane. CD11b is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-16), which is cleaved off in the mature form. It is predominantly expressed in monocytes and granulocytes and some expression is also observed in neutrophils. It is usually found as a heterodimer of alpha and beta subunits and associates with Integrin beta-2 (ITGB2). Two isoforms of CD11b have been reported that are produced by alternative splicing. CD11b contains a large extracellular domain (aa 17-1104), a short transmembrane domain (aa 1105-1128), and a cytoplasmic domain (aa 1129-1152). It has been implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It contains a metal ion-dependent adhesion site (MIDAS) in the integrin A domain, which is essential for stabilization of domain A in high affinity state. The major ligand recognition site of CD11b/CD18 has been mapped to the A-domain in the CD11b subunit (CD11bA). Both magnesium and calcium ions exhibit equal affinities for the inactive CD11bA, however, upon activation there is a 10-fold increase in the binding affinity of magnesium ions without affecting affinity of calcium ions. Mutations in ITGAM gene are known to cause Systemic lupus erythematosus 6, which is characterized by wide range of system dysfunctions, principally involving skin, joints, kidneys and serosal membranes. (Ref.: Zerria, K., et al. (2006). Immunology 119(4); 431-440; Mahalingam, B., et al., (2011). J. Immunol. 187 (12); 6393-6401; Li, R., et al. (2002). J. Immunol. 168 (3); 1219-1225.).

特異性

Clone mAb 107 detects CD11b/CD18 in human neutrophils. It targets an epitope with in the A domain.

免疫原

GST-tagged recombinant fragment corresponding to 211 amino acids from the N-terminal half of human CD11bA.

アプリケーション

Research Category
細胞骨格

Anti-CD11b/CD18, clone mAb107, Cat. No. MABF2085, is a mouse monoclonal antibody that detects CD11b/CD18 nd has been tested for use in Dot Blot, Flow Cytometry, Immunohistochemistry, and Inhibition assay.

Dot Blot Analysis: A representative lot detected CD11b/CD18 in Dot Blot applications (Li, R., et. al. (2002). J Immunol. 168(3):1219-25).

Flow Cytometry Analysis: A representative lot detected CD11b/CD18 in Flow Cytometry applications (Mahalingam, B., et. al. (2011). J Immunol. 187(12):6393-401; Li, R., et. al. (2002). J Immunol. 168(3):1219-25).

Inhibition Analaysis: A representative lot prophylactically inactivated CD11b/CD18 and prevents CD11b/CD18 binding to multiple ligands (Dehnadi, A., et. al. (2017). Nat Commun. 8:13899; Li, R., et. al. (2002). J Immunol. 168(3):1219-25).

Immunohistochemistry Analysis: A representative lot detected CD11b/CD18 in Immunohistochemistry applications (Dehnadi, A., et. al. (2017). Nat Commun. 8:13899).

品質

Evaluated by Flow Cytometry in human neutrophils.

Flow Cytometry Analysis: 1 µg of this antibody detected CD11b/CD18 in one million human neutrophils.

ターゲットの説明

127.18 kDa calculated.

物理的形状

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to lot specific datasheet.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.