MABE365

Anti-acetyl PARP1 Antibody, clone E4

clone E4, from mouse

• 別名: Poly [ADP-ribose] polymerase 1, PARP-1, ADP-ribosyltransferase diphtheria toxin-like 1, ARTD1, NAD(+) ADP-ribosyltransferase 1, ADPRT 1, Poly[ADP-ribose] synthase 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: E4, monoclonal
• application: WB
• 化学種の反応性: human
• テクニック: western blot: suitable
• citations: 1

特性

• 由来生物: mouse
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: E4, monoclonal
• 化学種の反応性: human
• テクニック: western blot: suitable
• アイソタイプ: IgG2bκ
• NCBIアクセッション番号: NP_001609
• UniProtアクセッション番号: P09874
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: acetylation (not specified)
• 遺伝子情報: human ... PARP1(142)

詳細

詳細

Poly (ADP-ribose) polymerase 1 (PARP1) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair. As a marker for apoptosis, PARP is cleaved in vitro by many caspases and in vivo by Caspase-3. Acetylation of PARP1 by p300/CREB-binding protein is important in the regulation of NF-kappaB-dependent gene activation through enhanced functional interactions with p300 and the Mediator complex.

免疫原

Linear peptide corresponding to acetylated human PARP1, short fragment.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
エピジェネティクス

Anti-acetyl PARP1 Antibody, clone E4 is a highly specific mouse monoclonal antibody & that targets PARP & has been tested in western blotting.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected wild type acetyl-PARP 1, but did not react with four acetylation-deficient PARP1 KQR mutants in WB (Messner, S., et al. (2009). The FASEB Journal. 23:3978-3989).

品質

Evaluated by Western Blotting in acetylated PARP1 fragment (373-529) purified protein lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected acetyl PARP1 in 10 µg of acetylated PARP1 fragment (373-529) purified protein lysate.

ターゲットの説明

~ 45 kDa observed. Both proteins we provided were acetylated in vitro by p300 using acetyl-CoA. While the short fragment (bacterially expressed) is very nicely acetylated, the full-length (bacolu system) is only weakly modified. The detection of the full-length protein is thus much more difficult.

物理的形状

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Acetylated PARP1 fragment (373-529) purified protein lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable