MABE290

Anti-Estrogen Receptor β Antibody, clone 9.88

clone 9.88, from mouse

• 別名: Estrogen receptor beta, ER-beta, Nuclear receptor subfamily 3 group A member 2
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 9.88, monoclonal
• 化学種の反応性: human, mouse, rat
• テクニック: immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgMκ
• NCBIアクセッション番号: NP_001035365
• UniProtアクセッション番号: Q92731
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ESR2(2100)

詳細

詳細

Estrogen receptor beta (ERβ; NR3A2 or ESR2) is a nuclear steroid receptor that has been detected in the ovary, testis, thymus, spleen, bone, brain, and kidney. It is a multidomain protein containing A/B, C, D, E, and F domains, and it is activated by estrogen, resulting in homo- or heterodimerization (with ERα). The active ERβ-estrogen complex binds to regulatory regions known as estrogen response elements on target genes; however, ERβ may also regulate transcription without a bound ligand via its activation function (AF-1) region in the A/B domain. The ERβ receptor interacts with a wide variety of proteins including transcriptional corepressors and coactivators that regulate its activity. Its expression is also regulated by hypermethylation of CpG islands in the ERβ promoter. Previous studies have reported that ERβ has anti-inflammatory and anti-proliferative effects, and may therefore be an important therapeutic target for cancers, especially those occurring in the reproductive organs.

免疫原

Recombinant protein corresponding to human Estrogen Receptor β.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

Research Sub Category
細胞周期、DNA複製及び修復

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Estrogen Receptor β in HUVEC cells (Simoncini, T., et al. (2006). Mol Endocrinol. 20(8):1756-1771.).

Use Anti-Estrogen Receptor β Antibody, clone 9.88 (Mouse Monoclonal Antibody) validated in WB, IP to detect Estrogen Receptor β also known as Estrogen receptor beta.

品質

Evaluated by Western Blot in PC-12 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Estrogen Receptor β in PC-12 cell lysate.

ターゲットの説明

~55 kDa observed. Uniprot describes eight different isoforms ranging from ~36 kDa to ~59 kDa

物理的形状

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
PC-12 cell lysate

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable