由来生物
mouse
品質水準
抗体製品の状態
culture supernatant
抗体製品タイプ
primary antibodies
クローン
7E6.2, monoclonal
化学種の反応性
human
テクニック
western blot: suitable
アイソタイプ
IgG1κ
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
ambient
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... SPRY1(10252)
詳細
Protein sprouty homolog 1 (UniProt O43609; also known as Spry-1) is encoded by the SPRY1 gene (Gene ID 10252) in human. Sprouty was originally identified in Drosophila as a negative regulator of fibroblast growth factor (FGF) signaling during tracheal development. There exist four mammalian homologs (SPRY1-4) that likewise mediate feedback inhibition against growth factor-/receptor tyrosine kinase- (RTK-) dependent Ras/mitogen-activated protein (MAP) kinase signaling pathway. Loss of function studies in mice show that SPRY1 and SPRY2 play critical roles during early development of multiple organ rudiments, including external genitalia, eye, kidney, inner ear, tooth, and cortical patterning in the brain. In addition, evidences indicate that SPRY1 plays an antiproliferative and tumor-suppressing role in cancers. SPRY1 downregulation is reported in various cancer types, including prostate, breast, and ovarian cancers, and SPRY1 expresion has been proposed as a marker of good prognosis in patients with renal cell carcinoma.
特異性
Clone 7E6.2 detects the ~35 kDa human SPRY1 by targeting an N-terminal region epitope.
免疫原
GST-tagged recombinant human SPRY1 N-terminal fragment.
アプリケーション
Research Category
幹細胞研究
幹細胞研究
This mouse monoclonal Anti-SPRY1 Antibody, clone 7E6.2, Cat. No. MABD390 is validated for use in Western Blotting for the detection of SPRY1.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected SPRY1 in 10 µg of SK-MEL-2 human skin melanoma cell lysate.
品質
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this hybridoma culture supernatant detected SPRY1 in 10 µg of HepG2 human hepatocellular carcinoma cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this hybridoma culture supernatant detected SPRY1 in 10 µg of HepG2 human hepatocellular carcinoma cell lysate.
ターゲットの説明
~35 kDa observed. 35.12 kDa calculated.
物理的形状
Unpurified
Mouse IgG1 hybridoma culture supernatant without azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
その他情報
Concentration: Please refer to lot specific datasheet.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABD390:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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