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詳細
Sonic hedgehog protein (SHH) or alternatively known as HHG-1, is a very important developmental intercellular signaling factor. SHH protein is cleaved into to active forms, an N-terminal product that stays associated with the cell membrane or forms freely diffusible multimers and a C-terminal form that diffuses into the extracellular matrix. SHH is expressed in fetal intestine, liver, lung and kidney but not normally in adult tissues except during some disease state like cancerous tumors. The intercellular signaling triggered by SHH is essential for a variety of patterning events during development including the development of the notochord, neural tube and plate, as well as the polarizing of the anterior-posterior axis of developing limb buds. Because of its importance in patterning during development, defects in SHH are the cause of a number of abnormalities including structural eye and brain abnormalities, teeth and bone malformations and finally limb and bone developmental defects. EMD-Millipore’s Anti-SHH mouse monoclonal antibody has been tested in western blot against recombinant antigen and cell lysates from HepG2, PANC-1, HeLa, SK-N-SH, F9, NIH3T3 and COS7 cells and in paraffin embedded immunohistochemistry of human liver cancer tissue sections. The antibody has also been successfully tested by flow cytometry against HeLa cells in culture.
免疫原
Purified recombinant fragment of human SHH expressed in E. Coli.
アプリケーション
Research Category
幹細胞研究
幹細胞研究
Research Sub Category
多能性及び初期分化
多能性及び初期分化
Anti-SHH Antibody, clone 5H4 is a highly specific mouse monoclonal antibody, that targets Sonic Hedgehog & has been tested in western blotting, Flow Cytometry & IHC.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected SHH in liver cancer tissue.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected SHH in non serum starved HeLa cells.
Optimal working dilutions must be determined by end user.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected SHH in non serum starved HeLa cells.
Optimal working dilutions must be determined by end user.
品質
Evaluated by Western Blotting in LNCaP, HepG2, PANC-1, HeLa, SK-N-SH, F9, NIH3T3, COS7 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected SHH in LNCaP, HepG2, PANC-1, HeLa, SK-N-SH, F9, NIH3T3, COS7 cell lysates.
Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected SHH in LNCaP, HepG2, PANC-1, HeLa, SK-N-SH, F9, NIH3T3, COS7 cell lysates.
ターゲットの説明
~50 kDa observed. Uncharacterized bands may appear in some lysate(s).
物理的形状
Protein G Purified
Format: Purified
Purified mouse monoclonal in PBS with up to 0.1% sodium azide and 0.05% protein stabilizer.
保管および安定性
Stable for 1 year at 2-8°C from date of receipt.
アナリシスノート
Control
LNCaP, HepG2, PANC-1, HeLa, SK-N-SH, F9, NIH3T3, COS7 cell lysates
LNCaP, HepG2, PANC-1, HeLa, SK-N-SH, F9, NIH3T3, COS7 cell lysates
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MABD175:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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