おすすめの製品
由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
28C4, monoclonal
化学種の反応性
human, rat, guinea pig, monkey
メーカー／製品名
Chemicon®
テクニック
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
アイソタイプ
IgG
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
wet ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... CHAT(1103)
rat ... Chat(290567)
rhesus monkey ... Chat(709977)
特異性
Reacts with Choline Acetyltransferase (ChAT).
免疫原
Purified human placenta ChAT.
アプリケーション
Research Category
ニューロサイエンス
ニューロサイエンス
Research Sub Category
神経伝達物質及び受容体
神経細胞及びグリアマーカー
神経伝達物質及び受容体
神経細胞及びグリアマーカー
Anti-Choline Acetyltransferase Antibody, clone 28C4 detects level of Choline Acetyltransferase & has been published & validated for use in ELISA, IH & WB.
Western blotting: 1:1,000-1:10,000 using a high sensitivity ECL kit. Recognizes a protein of ~67 kDa. May also recognize a protein of 35 kDa which is thought to be a degradation product. Suggested dilution buffer is TBS containing 5% skim milk powder and 0.05% Tween 20.
Immunohistochemistry on free-floating frozen sections of Rhesus monkey brain fixed with 4% paraformaldehyde and coronal vibratome sections of rat brain fixed with 4% paraformaldehyde and 0.2% glutaraldehyde.
ELISA (two side)
Optimal working dilutions must be determined by end user.
Immunohistochemistry on free-floating frozen sections of Rhesus monkey brain fixed with 4% paraformaldehyde and coronal vibratome sections of rat brain fixed with 4% paraformaldehyde and 0.2% glutaraldehyde.
ELISA (two side)
Optimal working dilutions must be determined by end user.
物理的形状
Format: Purified
Purified immunoglobulin. Liquid.
保管および安定性
Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
その他情報
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法的情報
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAB5350:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Zhaoqin Huang et al.
Molecular medicine reports, 11(5), 3285-3290 (2015-01-17)
The present study was conducted to investigate the 11C‑Choline metabolic mechanism and examine the association between 11C‑Choline metabolism and uptake in different pathological types of lung cancer. A total of 18 tumor specimens and corresponding normal lung tissues were collected
Katarina Zvarova et al.
The Journal of comparative neurology, 489(4), 501-517 (2005-07-19)
We examined the distribution and fate of cocaine- and amphetamine-regulated transcript peptide (CARTp)(55-102)-immunoreactive (IR) structures in the neonatal and adult rat urinary bladder. Double-labeling studies examining CARTp with tyrosine hydroxylase (TH), neuronal nitric oxide synthase (nNOS), or choline acetyltransferase (ChAT)
ELISA methods to measure cholinergic markers and nerve growth factor receptors in cortex, hippocampus, prefrontal cortex, and basal forebrain from rat brain.
Gearhart, Debra A, et al.
Journal of Neuroscience Methods, 150, 159-173 (2006)
Hanqing Tang et al.
International heart journal, 62(3), 647-657 (2021-05-18)
Bioinformatics analysis showed that miR-448-5p expression in the myocardial tissue of rats with myocardial infarction significantly increased, suggesting that it may participate in myocardial cell apoptosis in myocardial infarction. This study aimed to explore the protective effects of miR-448-5p on
Ji-Woon Kim et al.
PloS one, 9(8), e104927-e104927 (2014-08-19)
Autism spectrum disorder (ASD) is a group of pervasive developmental disorders with core symptoms such as sociability deficit, language impairment, and repetitive/restricted behaviors. Although worldwide prevalence of ASD has been increased continuously, therapeutic agents to ameliorate the core symptoms especially
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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